View Single Post
Mar2-10, 02:46 AM
Sci Advisor
PF Gold
Monique's Avatar
P: 4,642
Quote Quote by Ouabache View Post
In your experiment, are you allowed to put more than one protein per well?
It appears that space is constrained to three 384-well plates, but not time. Can we assume, you have time to incubate more than one plate?

In the o.p., lets make an assumption that only a small dose is necessary, to test positive for lethality (e.g. 1 ul) of wine. The line "You need to determine the answer after 24 hours"
is to be interpreted that, you need to find out the answer at the conclusion of 24 hours.
So time is a constraint. You only have time to run one experiment.
The experiment would be constrained in that you can only test 1152 reactions (3x384). You can put more than one protein in a well, but you need to come up with the most cost-effective way to identify the protein that interacts.

The O.P. is a tricky question, because it says that a sip is lethal after about 24 hours. This does not mean that you can do a single experiment in those 24 hours, because toxicity is in the dose. You could give the rabbits a higher dose and see a result quicker, but I am not sure how the rabbits would react to a higher dose of alcohol. That might be lethal to them as well.