CHEF Gel Troubleshooting: Help With Plugs & Ladder

[MatthewHaas]

Has anyone ever ran a CHEF gel before? I am having trouble getting my plugs to leave the wells. Ethidium bromide staining shows a thick band in the well, and I am supposed to have a ladder. I have some images I can post, but I figured it makes more sense to first see if anyone out there has any expertise in the area.

Thanks!

 

[chemisttree]

Did anything get out of the well? I assume that you have already ruled out power supply issues, yes?

 

[MatthewHaas]

I am sorry for the late reply. No, nothing got out of the well. We have not rulerd out power supply issues.

 

[chemisttree]

Power supply is the first thing you check. Use a voltmeter unless the power supply applies a pulsed potential. If it is pulsed, you need an oscilliscope.