CHEF Gel Troubleshooting: Help With Plugs & Ladder

  • Thread starter Thread starter MatthewHaas
  • Start date Start date
Click For Summary
The discussion centers on issues encountered while running a CHEF gel, specifically the failure of DNA plugs to migrate out of the wells. The user reports seeing a thick band in the well during ethidium bromide staining and mentions the absence of a ladder. Responses emphasize the importance of checking the power supply, suggesting the use of a voltmeter to ensure proper function. If the power supply is pulsed, an oscilloscope may be necessary for troubleshooting. The conversation highlights the need to rule out power supply issues as a primary step in resolving the migration problem.
MatthewHaas
Messages
15
Reaction score
0
Has anyone ever ran a CHEF gel before? I am having trouble getting my plugs to leave the wells. Ethidium bromide staining shows a thick band in the well, and I am supposed to have a ladder. I have some images I can post, but I figured it makes more sense to first see if anyone out there has any expertise in the area.

Thanks!
 
Biology news on Phys.org
Did anything get out of the well? I assume that you have already ruled out power supply issues, yes?
 
I am sorry for the late reply. No, nothing got out of the well. We have not rulerd out power supply issues.
 
Power supply is the first thing you check. Use a voltmeter unless the power supply applies a pulsed potential. If it is pulsed, you need an oscilliscope.
 
Thread 'Magnetoreception in Animals'

Thread 'Magnetoreception in Animals'

For more than a hundred years people have been intrigued by how animals are able to do certain navigating tasks so well. Being able to sense magnetic fields has been one of several clues animals could use to figure out where they should go. Among possible magnetic sensory mechanisms have been: light sensitive proteins (cryptochromes) in the retina that can also react to magnetic fields. Microscopic magnetite crystals on found in various areas of the body (often the nose near nerves) A new...
View full post »

Similar threads

Troubleshooting: Imaging Agarose Gel with Ethidium Bromide
  • · Replies 5 ·
Replies
5
Views
7K
Plumbing Water Softener Troubleshooting/Repair
  • · Replies 8 ·
Replies
8
Views
4K
Mechanics of Materials -- inclined ladder
  • · Replies 9 ·
Replies
9
Views
4K
Giant Hogweed (Heracleum mantegazzianum) - dangerous plant
  • · Replies 3 ·
Replies
3
Views
2K
Protocol and solvent help needed for gel-permeation chromatography
  • · Replies 11 ·
Replies
11
Views
6K
Jacob's Ladder construction help.
  • · Replies 4 ·
Replies
4
Views
2K
I Solving a momentum problem where masses change after the collision
  • · Replies 35 ·
2
Replies
35
Views
3K
Arduino and Chromebook (Plug-in)
  • · Replies 2 ·
Replies
2
Views
2K
RF testing: Balistics Gel for human analog?
  • · Replies 5 ·
Replies
5
Views
3K
How Do You Calculate Frictional Force and Slippage on a Ladder?
  • · Replies 2 ·
Replies
2
Views
8K