Recent content by sobored

Thanks for helping me out. Both groups have 35 samples and the values are continues. Can i use student T test to predict this p-value, assumming that i use the parametric way?

I have made a correlation between group A and B and the pearsson coefficient constant, r, is at 0.97. The values are expression levels and hence they are non-parametric. I wonder how can i calculate the p-value of this correlation between group A and B? I know how to calculate the p-value for...

Can someone please tell me what is the difference of non-canonical and canonical DNA segments? I have searched for in the net, but have not got any good explanations of these names. Thank you a lot!

I am looking for any articles with references talking about WESTERN BLOTTING, but i hardly find any in pubmed. Could it be that WESTERN BLOTTING is an old technique that is why old articles are deleted or not in use? Do you have any good ones that i can read? I am not interested in some of...

Anyone has any good and useful Discussion forums for microarray? I appreciate it a lot! Thank you.

How to make 50%v/v and 50%w/v glycerol? What is the difference? Glycerol/glycerin has a density of 1.25 gm/mL. Thanks!

Okey, i am confused about this matter. I have two different samples. One is 5 untreated/control samples (same kind) and the other is a 50 treated samples (same kind). I wonder what kind of statistic methods i can use to compare the results between them? I don't think that i can use ANOVA...

Thanks for your answer! I still have some questions regarding this search tool. Let us take for example the FOS gene for the human. I came to this site: http://www.ncbi.nlm.nih.gov/entrez/query.f...&list_uids=2353 I am now overwhelmed with all the infos and sequences in this site...

Height: 5'1 Weight:105 What is this height in metres and this weight in kg?

Search tools for ... Where and how to get the gDNA, mRNA and cDNA sequences of a gene? Any search tools? Thanks!

When I use Primer3 design tool and the default Primer3 uses [Mg++] = 1.5 mM, whereas the average concentration is 5 mM in PCR kits. This concentration has a huge effect on the Tm. There is a “Salt concentration” box. Testing primer set designs for accuracy has shown that making the “Salt...

Anyone who has problem in signing up in Hotmail account? I can't check my mails!

I wonder do the genes have the same exon-intron junction sequences or do they have different junction sequences? I was told that all genes have this general junction sequences of the exon-intron-exon: 5'---exon---A/CG-><-GUPuAGU----intron-----Py12NPyAG-><-G---exon---3' The arrows indicate...

It says that i have to pick primers between 17-21 bp which are found in the last half of the cDNA. How come the last part of the cDNA? Thanks.

The protocol says that i have to air-dry the isolated RNA. Would not this contaminate the sample with RNase and damage my RNA sample? Thanks.