Proteins Definition and 72 Threads

  1. K

    How Does S-Layer Protein-Based Data Storage Compare to Traditional Hard Disks?

    An S-layer protein is 3nm is diameter. Calculate number of proteins per mm3 of membrane. If the protein is used to pattern a surface used for data storage - each protein molecule can position one magnetic particle (1bit) compare this type of storage with current hard disks. Just one...
  2. P

    Iso-electric point of proteins?

    I'm crystallizing apoferritin, and still don't understand what an iso-electric point is, despite reading a whole chapter of a book about it. What happens if the surrounding buffer solution is at the iso-electric point? Cheers!
  3. C

    Milk analysis? investigate the proteins

    For protein analysis, why is milk sample neutralised before the addition of formalin in Formol titration? could we say that... The proteins are too weak to be titrated directly with alkali,if formalin is added, it reacts with the -NH2 groups to form the methylene-amino(-N=CH2) group and...
  4. A

    The central dogma: proteins first?

    I recently borrowed the book Information Theory, Evolution, and the Origin of Life by Hubert Yockey. In it he points to the fact that there can be no code from the amino acid alphabet (about 20 symbols) to the RNA/DNA alphabet (64 3-letter codons, a second extension of the 4-symbol alphabet...
  5. L

    Help with Questions on Membrane Structure, Proteins & Vitamin E

    I have some questions that i am not sure...can u guys help me out ? 1. Which of the following is the best phrase to describe the modern view of membrane structure? a. Membranes are lipid-lipid bilayers. b. Membranes are lipid-lipid bilayers with associated proteins. c. Membranes are...
  6. L

    Difference between fibrous proteins and globular proteins

    Hey guys, can somebody helps me to understand better about fibrous vs globular protein please "So what is the main difference between fibrous proteins and globular proteins? Aside from the difference in shape (elongated vs. spheroidal) and solubility (insoluble vs. soluble), fibrous proteins...
  7. J

    Using Biuret Solution to Quantitatively Measure Proteins

    hi all! I was just thinking about organic compound indicators, and biuret solution for proteins crossed my mind. I know that it can be used to qualitatively tell the presence of proteins (peptide linkages) with a colour change. But is there a way that biuret solution can be used to take...
  8. M

    Can goats be genetically modified to produce medicinal proteins?

    Like as far as i know. They can be designed so they make certain proteins. So this allows goats to create proteins that are needed for insulin or the proteins for bulletproof vests. But say they are making proteins that are used in the body like insulin does this affect the goat's system at...
  9. N

    Hybrid protein which is composed of two proteins

    If you have a hybrid protein which is composed of two proteins that are very different from each other, will you be way less likely to be able to make the hybrid protein retain the original functions of one of the proteins/either of the proteins because of that? Thanks, Sorry for bothering you
  10. S

    Protein samples still in liquid form at -20C, Storage condition for proteins

    Dear folks, Please help me out. I store my purified protein samples at -20C and they are still at the liquid form (a few are frozen) at this temperature! :cry: I used Trizol kit to purify them and dissolved them in a solution of 9M urea, 4% CHAPS and 30mM Tris/HCl pH 8.5 buffer. They had...
  11. S

    Verifying Sizes of Human Pituitary Proteins

    Can anyone please help me to find the sizes (Da) for these human pituitary proteins? i have looked for them in Entrez and other bioinformatic sites, but am really not sure if they are right. Please, recheck them for me. Thanks a bunch! 1. Glycoprotein hormones alpha chain i have a vERY...
  12. S

    Purification of proteins from human tissue, SDS_PAGE

    I plan running SDS-PAGE to get the protein profile of human tissues. Which kit is best for this purpose and how can i measure the concentration of a mixture of different proteins? I can measure the protein concentration when it is just a single kind of protein in the mixture, but how about when...
  13. C

    Secondary/ tertiary structure of proteins

    Why does the secondary/ tertiary structure of a protein exist? Is it due to the fact that all the bonds in polypeptide are not straight as we tend to draw them? ie - bond angle, such as the 109.5 degrees for C-C, exist and also bonds are free to rotate unless hydrogen bonds are present. (as they...
  14. H

    Building blocks of proteins are the beta-amino acids

    Which of the following are true ? (a) Proteins range from a MW from about 3,000 to 1.0e6 g/mol (b) Fibrous proteins are the main component of Muscle,hair and cartilage (c) Building blocks of proteins are the beta-amino acids (d) Polar amino acid side...
  15. B

    Where Are Proteins Synthesized and Routed in a Cell?

    I have been asked to describe the pathway the following types of proteins follow and where they are synthesized in the cell. (a) Soluble proteins meant to remain in cytoplasm- Here wouldn't this follow the pathway cytoplasm-ER-golgi-exocytosis\ (b) membrane proteins meant to remain...
  16. D

    Unravelling the Mystery of DNA, RNA and Proteins

    Are the whole processes of DNA, RNA, ribosomes, amino acids and proteins all just "chemical reactions" or is there something more to it? I know the way it works from DNA to protein but why? I understand this question might have a very complex anwser but a brief explanation would be...
  17. B

    Understanding DNA Binding Proteins: Mechanisms of Sequence Recognition

    In general how do DNA-Binding proteins bind to DNA? Many DNA-binding proteins bind to specific sequences of DNA bases. How do the proteins accomplish this sequence recognition. My answer: In general, DNA binding proteins possesses certain structural motifs, such as helixes, which enable...
  18. B

    How Do Antifreeze Proteins Help Antarctic Fish Survive the Cold?

    Anyone know the answer to this ridiculous problem Antarctic notothenioid fish avoid freezing in their perpetually icy environment because of an antifreeze protein that circulates in their blood. This evolutionary adaptation has allowed these fish to rise to dominance in the freezing southern...
  19. E

    Unraveling How Proteins Are Transported & Sorted in Cells

    Hi! If I could get an answer to this question I could understand biology a lot easier. In the cell interior, the endoplasmic Reticulum manufactures proteins and are then transported by a vesicle to the Golgi Apparatus where they are modified or processed and sorted. My question is, ''how do...
  20. V

    AGCTU, and all-important proteins

    <1> Can anyone tell me where these alphabets come from ? I mean who first used them to talk about genes' base ? <2> All life on Earth is proteins but is there any life without proteins ? Would you, biologists please tell me how you study enzymes in your labs ? I am not majoring in biology, I...
  21. FZ+

    Denaturising Proteins: Mechanism & Effects Explained

    Exactly what is the mechanism that makes proteins denature under certain conditions? (eg. too high temperature, pH etc etc) How does it all work?
  22. STAii

    Transport Proteins in Plasma Membrane

    Greetings. According to my textbook (again, this might not be really accurate because of translation): Now, here is my question : If proteins are just molecules (big ones i guess), how do they 'know' what the cell needs at a certain time (to let it into the cell) ? Thanks in advance.
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