Nice little paper about properly validating your reagents: http://www.ncbi.nlm.nih.gov/pubmed/22361696 Why is it in academia absolutely no one validates their reagents like antibodies while in industry it is absolutely required? Rather than admitting their results are due to nonspecific binding, I've seen some papers where academics attribute their results to say something like another unknown isoform of the protein that they're studying. Why is it that the peer review system has completely and utterly failed to to address this type of terrible science? What's worse is that once results that come from an antibody that might not be specific is published, many other labs go out and start using the same antibody to study the protein(s) in question. What a complete an utter joke a lot of science being done now has become. Manufacturers are notorious for being lazy when it comes to validating their antibodies, academics simply take it for granted that they are supposed to work. Maybe it is time our universities do high quality science with validation like industry?