Amino acid biosynthesis from CAC intermediates

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SUMMARY

The discussion focuses on the conversion of furamate and citrate into amino acids using transaminase and liver homogenate, with alanine as the amino donor. The participant successfully identified that citrate is converted to oxaloacetate, which then undergoes transamination to form aspartate. Similarly, furamate is converted to α-ketoglutarate and subsequently becomes glutamate. The participant emphasizes the importance of recognizing straightforward solutions in experimental processes.

PREREQUISITES
  • Understanding of the citric acid cycle (CAC) and its intermediates
  • Knowledge of transamination reactions and their mechanisms
  • Familiarity with amino acid biosynthesis pathways
  • Experience with using liver homogenate in biochemical experiments
NEXT STEPS
  • Research the mechanisms of transaminase enzymes in amino acid synthesis
  • Study the role of α-ketoglutarate in metabolic pathways
  • Explore the biochemical properties of liver homogenate for enzyme reactions
  • Learn about the conversion processes of other CAC intermediates into amino acids
USEFUL FOR

Biochemists, molecular biologists, and researchers involved in metabolic engineering or amino acid biosynthesis will benefit from this discussion.

Jikx
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Hello,

I'm having a bit of trouble with an experiment. It involves converting furamate and citrate into amino-acids using a transaminase. We are using a liver homogenase, with the amino-donor being alanine.

However, whatever resource i use, furamate/citrate is not converted into an animo-acid!

Any suggestions? THANKS!

(my experiment failed, but I still to write up the discussion!)
 
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I worked it out.. kind of.

Citrate is converted to oxaloacetate (via CAC), then undergoes a transamination reaction to Aspartate.

Furamate is converted to a-ketoglutarate (also via CAC), then becomes Glutamate.

Its just a curse with me... always ignore the simplest answer because its too simple.
 

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