Bear with me if you notice any botched ideas in my writing, I'm working on it. Initially I was wondering about direct injection of ATP. But I ran into a roadblock... First - Is it possible to isolate ATP from a source and make a high concentration ATP solution or even pure ATP? (extraction method, solvents, etc.) YES http://www.ncbi.nlm.nih.gov/pubmed/19009538 Second - With this ATP what are the effects of a direct injection into tissues or from intravenous drip or injection. My question is; what would happen if an excess amount of ATP were suddenly present locally in the brain, heart, abdomen, or muscle? Also, how would ATP in an IV affect you? I do not think (but please check me if I am wrong) that ATP is transported in the blood plazma. I do not know if ATP is transported between cells at all or just intracellularly, it makes sense that it would require vesicle transport. Vesicle transport makes sense because - EDITED - ATP is not stable enough to be transported, and synthesis and consumption are closely linked. "...cell membranes are [generally] impermeable to ATP..," http://resources.metapress.com/pdf-preview.axd?code=n134561068312v36&size=largest Basically injecting pure ATP would not affect the interior of cells, since the vast majority would lose the potential energy stored in the phosphate bond before it reached a suitable target site to activate something. My original question is null. However, an upgraded version of my question - what happens if extremely high levels of ATP were imposed upon a normal cell?