Biochemistry - Calvin Cycle Question

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SUMMARY

The discussion centers on the effects of light cessation on the levels of 3-phosphoglycerate (3-PG) and Ribulose 1,5-Bisphosphate (RuBP) in the Calvin Cycle. It is established that while light-dependent reactions produce ATP and NADPH necessary for RuBP synthesis, 3-PG can still be produced through carbon fixation. Therefore, when light is switched off, RuBP levels decrease due to the lack of ATP and NADPH, while 3-PG levels may initially increase as carbon fixation continues despite the absence of light.

PREREQUISITES
  • Understanding of the Calvin Cycle and its components
  • Knowledge of light-dependent and light-independent reactions in photosynthesis
  • Familiarity with the roles of ATP and NADPH in biochemical processes
  • Basic concepts of carbon fixation and its mechanisms
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  • Study the role of ATP and NADPH in the Calvin Cycle
  • Research the process of carbon fixation and its regulation
  • Examine the enzymatic reactions involved in the Calvin Cycle
  • Explore the impact of light intensity on photosynthetic efficiency
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Students of biochemistry, educators teaching photosynthesis, and researchers interested in plant physiology and metabolic pathways.

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Homework Statement



An illuminated suspension of chloroplasts is actively carrying out photosynthesis. What would happen to the levels of 3-phosphoglycerate and Ribulose 1,5-Bisphosphate if the light was suddenly switched off?


Homework Equations





The Attempt at a Solution



I thought that the answer would be that the levels of 3-phosphoglycerate and Ribulose 1,5-Bisphosphate would decrease because the CO2 that is fed into the Calvin Cycle to make these compounds comes from the light-dependent reactions. Also, 3 of the enzymes in the stroma that regulate the Calvin Cycle are light-dependent as well.

However, someone told me that the 3-phosphoglycerate levels would increase instead. I was wondering if someone could please tell me why this would happen?

Thanks in advance!
 
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persephone said:
the CO2 that is fed into the Calvin Cycle to make these compounds comes from the light-dependent reactions.

This statement is incorrect. There are two important molecules that are produced by the light-dependent reactions. What are they and how might removing them affect the Calvin cycle?
 
Ygggdrasil said:
This statement is incorrect. There are two important molecules that are produced by the light-dependent reactions. What are they and how might removing them affect the Calvin cycle?

Sorry, I don't know why I said that. The molecules that are produced by the light-dependent reactions are ATP and NADPH. I know that you need both to make Ribulose 1,5-Bisphosphate in the Calvin Cycle, so that means shutting off the light would decrease the levels of Ribulose 1,5-Bisphosphate.

However, ATP and NADPH are NOT needed to make 3-Phosphoglycerate. So I am still not sure how shutting off the lights would effect the levels of this compound...If you could please clarify this for me I would really appreciate it!
 
Think of the reactions that produce 3-PG and those that use up 3-PG. Which reactions are dependent on ATP and NADPH and which ones do not require these compounds?
 
had a question like this on bio exam. Remember that Carbon fixation is still occurring but the rest of the cycle is not.
 

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