Biochemistry Definition and 32 Discussions

Biochemistry or biological chemistry, is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells, in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenomena.Much of biochemistry deals with the structures, functions, and interactions of biological macromolecules, such as proteins, nucleic acids, carbohydrates, and lipids. They provide the structure of cells and perform many of the functions associated with life. The chemistry of the cell also depends upon the reactions of small molecules and ions. These can be inorganic (for example, water and metal ions) or organic (for example, the amino acids, which are used to synthesize proteins). The mechanisms used by cells to harness energy from their environment via chemical reactions are known as metabolism. The findings of biochemistry are applied primarily in medicine, nutrition and agriculture. In medicine, biochemists investigate the causes and cures of diseases. Nutrition studies how to maintain health and wellness and also the effects of nutritional deficiencies. In agriculture, biochemists investigate soil and fertilizers. Improving crop cultivation, crop storage, and pest control are also goals.

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  1. P

    Biology Ran GTPase (Biochemistry)

    Hi everyone! There is a question that I can't solve. Ran GTPase controls nuclear import of proteins and is present at a much higher concentration than its GAP (GTPase activating protein) and GEF (guanine nucleotide exchange factor) proteins. Imagine a mutation in the Ran GTPase resulting in an...
  2. FlyingWhale

    Biology Sketch the change of pH in the breakdown of proteins into amino acids

    The pH stays the same because the optimum pH of trypsin is around pH 7.5. I think??
  3. samy4408

    Medical Question about the synthesis of fatty acids

    Hello , in the course of fatty acids synthesis the professor said that after the repetition of the cycle 7 times we end up with a 16 carbon chain and he added that all the carbons came from acetyl-COA except for one . i didn't understand the last one , what is the carbon that don't come from...
  4. samy4408

    A question about nucleic acids

    Hello , is minor bases and Nucleoside the same thing ?
  5. samy4408

    Medical Hello, I found a weird sentence in a book about enzymes....

    Hello ! We all now that enzymes lower the activation energy by creating both covalent bonds and week non covalent bonds , in this book , about how the enzyme lower the activation energy using covalent bonds they said : this sentence is not compatible with a basic principle that i learned in...
  6. samy4408

    Something that doesn't make sense to me about calculating the pH

    Hello , the problem that i refer to is present when we try to calculate the pH of a solution without any assumption. in a lecture entitled "calculating the pH of a strong acid/base solution " after adding a certain amount of HCl in water we are asked to calculate the pH (the amount of HCl and...
  7. samy4408

    Question about lipids -- Glycerol and sphingosine form the backbone of the lipid?

    Hello , I am studying lipids and a point got me confused . it is said that beside the fatty acid we have glycerol and sphingosine that forms the backbone of the lipid . is the lipid formed by those 3 components at the same time (fatty acid +glycerol +sphingosine ) or it is formed by the fatty...
  8. Biochemgirl2002

    How to calculate error on log(MW) and error on MW for an SDS-PAGE calculation?

    I have found my LINEST equation for my standard curve, which is where y=log(MW) AND x= Rf y=-1.392047709x+2.324113743 as well, my imageLab gives me: Relative Front (Rf) = 0.087 error on Rf=0.005 I can calculate my log(MW) by inputing my Rf into x value of the equation...
  9. EFech

    Protein Folding Entropy

    I have been reading about protein thermodynamics and found different types and models for entropy calculation before and after protein folding. I understand Vibrational, conformational, configurational entropy are some of the most studied "types" of protein folding entropy. My questions is...
  10. Sophrosyne

    Medical Tensile strength of cell membranes

    The structure of the cell membrane is depicted as being formed from a bilayer of phospholipids with their hydrophilic portions pointing outwards and their hydrophilic portions facing each other. But as I look at the histology of the epithelial layer of the epidermis or mucous membranes, I am...
  11. S

    Question about antibody affinity chromatography

    I am learning about protein purification in my Biochemistry class, and I have a question about protein elution in antibody affinity chromatography. My understanding of the mechanism for the technique is that proteins that do not bind to the antibody will be separated first as it runs down the...
  12. C

    Anti-viral Activity of Anise Extract

    Anise extracts contain Lignin-Carbohydrate complexes (LCs), which have been found to have anti-viral effects according to this article (DOI: 10.1271/bbb.100645 ). However, from what I understood by going quickly through the article, the scientists applied the extract directly onto the cell...
  13. osg99

    Creating A Ligand // Selective Androgen Recepetor Modulator (OSTARINE)

  14. M

    Chemistry What is the ratio of pH=Pka-1?

    I think it is 1:10 but I am not sure the meaning of PKa -1, can someone explain it please.
  15. TytoAlba95

    Preparation of 1mg/ml BSA

    I had to prepare 1mg/ml BSA for Total protein estimation by Lowry method. I was suggested to add 1ml distilled water to 1mg of BSA in a 2 ml ependoff tube, instead of adding distilled water upto the 1ml mark. The explanation that was given to me was, that, 1mg is a very small amount and it...
  16. Sophrosyne

    The chemistry/physics of saltatory nerve conduction

    Saltatory nerve conduction occurs because of myelination of nerve fibers. This is done by Schwann cells in the peripheral nervous system and oligodendrocytes in the central nervous system. From what I understand, it happens because depolarization of the membrane at an unmyelinated area of the...
  17. M

    Electrophoresis of peptides

    Hi all, I have a question about this exercise: Which is the best option of pH for electrophoresis separation of two peptides with isoelectric points of 10.3 and 7 respectively? The given options of pH are 3,8 or 10. I've thought that if pH is more than 7, then, there will be positive charge...
  18. J

    Microbiological Fuel Cell: Negative Voltage

    Hello! I've been working on a few soil based MFCs and I have been monitoring my voltage and energy output for a week now. I've made the electrodes by covering stainless steel metal meshes with activated carbon using epoxy. I've gotten some funky results as attached. As you can see... the...
  19. Asmaa Mohammad

    Fischer projection of glucose

    Hello, my book shows this figure of Fischer formula of D-glucose I don't understand this figure, and I wonder why the upper carbon atoms in both the right and left formulas have 5 bonds. And from where comes this hedrogen atom in the upper left side of the figure (attached to alpha...
  20. H

    Volumes of a Buffer using Henderson-Hasselbalch?

    Homework Statement Calculate the volume of 0.10M phosphate solution to mix to prepare 100mL of a buffer with pH 6.0 starting with 0.10M stock solutions of NaH2PO4 and Na2HPO4. The pKa for this reaction is 7.21. Homework Equations pH=pHa+log( [A-]/[HA]) The Attempt at a Solution When I did it...
  21. E

    Codeine based biochemistry

    OK, in my story with a human colony ship headed to a new planet, I'm thinking of making the life on that planet originate from a comet carrying life with a shared ancestry to Earth life. Which means that they'll be a bit less alien than they otherwise could have been. They will share a lot of...
  22. T

    MCQ- Enzyme kinetics

    Homework Statement An enzyme catalysed reaction becomes zeroth order when: a.The velocity of enzyme action very low b. The velocity of enzyme action very high c.Enzyme conc very high d. All of these The attempt at a solution I understand An enzyme mediation recation becomes Zeroth order if...
  23. T

    Medical Biochemical reason behind (neurotic) perfectionism

    Is the reason behind neurotic perfectionism known? I'm looking for the molecular basis of this kind of perfectionism but couldn't find much on Google - Web and Scholar.
  24. Jimster41

    Oak Gall Wasp Mechanism

    How does the Gall Wasp get an Oak tree to make these? http://ento.psu.edu/extension/factsheets/galls-oak https://en.wikipedia.org/wiki/Gall_wasp I am not able to find anything other than "induced by chemicals secreted by the wasp" more or less. I have been looking at these over the last six...
  25. M

    Engineering Biomedical Engineering vs. Bioinformatics

    Which of these fields would allow one to make a bigger impact on treatment of mental illnesses? What about neurological disorders? And if you could also explain why that would be great, Thanks!
  26. B

    DNA Topology

    Homework Statement Suppose that a turn of B-DNA in a circular DNA molecule with L = 100 and W = -4 becomes a turn of Z-DNA . a) What are the L, T, and W following the transition? Homework Equations L=T+W L=#bp/#bp/turn B-dna= 10.4bp/turn Z-dna=12bp/turn The Attempt at a Solution...
  27. J

    A What is the best Quantum Model to solve many body problems?

    I know to describe Quantum Mechanical systems we can use: -Schrodinger equation -Feynman Path Integral method -Heisenberg Matrix formulation Well my question is, if you want to calculate molecular properties, and want to understand biochemistry (protein), you have a system with several...
  28. E

    Advice for Entering Biochem MSc./PhD after Phys. BSc.?

    I have a BSc. Honours Physics degree and I have summer research experience in Biophysics and Computational Chemistry. I want to enter a grad school program where I may use the basic physics that I've acquired throughout my undergrad degree, but where the focus is around biological function. The...
  29. Vannay

    Ideal advisor wary of my lack of knowledge in biochem....

    I'm in a physics graduate program shopping around for research advisors and I met with my number one pick today. He works with biological systems using calorimetry and other lab methods a biologist would know. He saw my enthusiasm and liked it but was wary about my lack of biochem knowledge. He...
  30. N

    Biochemistry Undergrad to Bioengineering PHD

    Hey! So I graduated with a BS in Biochemistry and I was originally considering medical school but after studying the horrible mcat for 3 years, I decided I had enough of that. Furthermore, I really lost interest in any aspect of patient work. However what has always interested me since I was a...
  31. N

    Programs Advantages of Majors/Minors

    Hi everyone, I have a question about the intricacies of Majors and Minors. Not sure if the topic/prefix is correct, any mod that thinks it should be under something else please go ahead and change it. But my question is regarding the specifics and impacts of majors and minors. I plan on...
  32. L

    Help with questions on becoming a surgeon?

    Im almost 16 and in 10th grade and I am very interested in biology/human anatomy. But when i was in 9th grade i was homeschooled and it wasnt for me and i ended up procrastinating to the point were i was kicked out in the middle of the second semester (but you need to finish the whole semester...
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