Biology; clarification of apple juice, help please

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SUMMARY

The discussion centers on the clarification of cloudy apple juice using pectinase and cellulase, specifically addressing the role of cellulase in breaking down cellulose microfibrils in apple cells. Participants confirm that cellulose remains suspended in the juice after cell degradation, necessitating the use of cellulase for effective clarification. The reference to "Processing Fruits: Science and Technology" by Diane M. Barrett provides authoritative insight into the composition of apple cell walls and their impact on juice clarity.

PREREQUISITES
  • Understanding of enzyme functions, specifically pectinase and cellulase.
  • Knowledge of plant cell structure, particularly cellulose and microfibrils.
  • Familiarity with the process of juice clarification.
  • Basic concepts of fruit processing and technology.
NEXT STEPS
  • Research the specific mechanisms of pectinase and cellulase in juice clarification.
  • Explore the role of cellulose in fruit cell walls and its impact on juice quality.
  • Study the techniques for optimizing juice clarification processes.
  • Investigate additional resources on fruit processing, such as "Processing Fruits: Science and Technology" by Diane M. Barrett.
USEFUL FOR

Students in biology or food science, food technologists, and anyone involved in fruit processing or juice production will benefit from this discussion.

Rose-Elise
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I'm doing my biology coursework on the clarification of cloudy apple juice using pectinase and cellulase. I've found loads of useful information on pectinase and exactly how it clarifies the juice but absolutely nothing on cellulase so I'm a tad stuck..

I know that the microfibrils in the apple cells are made of cellulose and I'm guessing that cellulose remains as a suspension once the cells have degraded, can anyone give me any further detail please?

Thank you!
 
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I don't know an answer to this one...have never done anything on clarifying apple juice. But, just at face value, your answer sounds reasonable. Cellulase breaking down cellulose, and a reasonable guess about what cellulose might be in cloudy apple juice that requires breaking down.
 
Thanks for your time & comments.

After a good hour searching through Processing Fruits: Science and Technology by Diane M. Barrett I finally found that it is indeed to do with the microfibrils in the primary cell wall of parenchyma cells in the skin and pulp of the apples.

Thanks again!
 

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