Calculating PFA Diffusion Rate in 1% Agar Gel: Tips and Resources

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harryfeng
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Hi:

I am doing a research project in which I need to diffuse PFA(Paraformaldehyde) into agar gel. I want to know how to calculate the diffusion rate. The agar gel is 1%, and 2 mm thickness. How long approximately does it take for PFA to diffuse from the top to the bottom?

Is there any article that I can read about?

Thanks
 
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I need to measure the diffusion rate of PFA in 1% agar gel.