Calculating the Percent of Cytosine in a DNA Molecule with 28% Thymine

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Discussion Overview

The discussion revolves around calculating the percentage of cytosine in a DNA molecule given that it contains 28% thymine. Participants explore the relationships between the different nucleotide bases in DNA and the implications of base pairing on the overall composition.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested, Homework-related

Main Points Raised

  • One participant asks if the percentage of cytosine can be determined solely from the 28% thymine, suggesting a possible answer of 72% without further justification.
  • Another participant emphasizes the need for additional information, questioning whether knowing only one base is sufficient for calculating the percentage of another base.
  • Several participants note the bonding relationships between the bases: thymine with adenine and cytosine with guanine, indicating that these relationships affect their percentages.
  • A participant suggests that if perfect Watson-Crick base pairing is assumed, the percentage of adenine would equal the percentage of thymine, allowing for a calculation of cytosine based on the total percentage of bases.
  • A later reply references a specific problem from a textbook, providing a detailed breakdown of base percentages in a double-stranded DNA molecule, indicating that the concentration of guanosine equals that of cytosine, thus providing a concrete example of how to approach the problem.

Areas of Agreement / Disagreement

Participants express differing views on whether the percentage of cytosine can be calculated from the given information. Some argue that additional information is necessary, while others propose that assumptions about base pairing can lead to a solution.

Contextual Notes

The discussion highlights the dependence on assumptions regarding base pairing and the total composition of nucleotides in DNA, which may not be explicitly stated in the initial question.

BBboy
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If a DNA molecule containes 28% thymine, what percent of it will be cytosine?

will it be 72%?

please help, thank you
 
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You'll have to show some work.

First, is there anymore information that comes with the question? There four different base pair that makes up DNA. Do you think knowing only one will help in solving the percentage of a certain base pair?
 
T - thymine bonds with A - adenine
C - cytosine bonds with G - guanine
how can you calculate the percentage of cytosine if it only gives you 28% thymine?
 
You know that thymine will only bond with adenine, so the percentages of each one must be related to each other somehow.
The same thing with cytosine and guanine.
 
Also assume that DNA is only composed of C,G,T and A. Therefore the total percentages of each individual base pair will equal 100 percent.
 
BBboy said:
T - thymine bonds with A - adenine
C - cytosine bonds with G - guanine
how can you calculate the percentage of cytosine if it only gives you 28% thymine?
You can't if that's all the information you're given. Are you sure you weren't given more information than that?
 
Actually if you assume perfect Watson-Crick base pairing, then all you need is the percentage of one base, since the base it bonds to will be the same percentage.

So the percentage of A = percentage of T
And 100- percentage of A + percentage of T = percentage of C and T

percentage of C = percentage of T = 1/2 percentage of C and T
 
This is a question from P. 232, #17:

http://www.emporia.edu/biosci/genetics/prob9.htm

P. 232, #17. A double stranded DNA molecule is 28% guanosine (G).
a. What is the complete base composition of this molecule?
In double stranded DNA the concentration of guanosine equals the concentration of cytosine, thus the molecule is also 28% cytosine. This leaves 44% for adenosine and thymine combined (1-28%-28% = 44%). As the concentration of adenosine equals the concentrations of thymine, the concentration of adenosine must be 22% and the concentration of thymine must also be 22%.

b. Answer the same question, but assume the molecule is double-stranded RNA.
For double stranded RNA the reasoning is the same except that uracil replaces thymine. Thus for double stranded RNA the base composition is 28% guanosine, 28% cytosine, 22% adenosine, and 22% uracil.
 

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