1. Not finding help here? Sign up for a free 30min tutor trial with Chegg Tutors
    Dismiss Notice
Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Can molecular complementation act as a protein inhibitor?

  1. Dec 5, 2008 #1
    I've always wondered about this. Bimolecular fluorescence complementation allows the incorporation of fragments of the green fluorescent protein (GFP) at the N and C-terminus of separate monomers. If the monomers dimerize, the process allows the GFP fragments to come together and form a functional unit that fluoresces, thereby confirming the existence of specific dimers.

    Is it possible to use the same method to introduce perhaps peptides or fragments of a small protein into separate monomers, whereby the dimerization process allows the formation of a functional unit recognizable by proteases or antibodies to inhibit the activity of the dimer?
  2. jcsd
  3. Dec 6, 2008 #2
    A very interesting and well articulated question. I have no answer and am as curious as you are. :)
  4. Dec 10, 2008 #3
    Would it not be easier to simply add a simple tag that can be used to cleave the dimer, once formed?
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?