Cell Biology - Devising Experiments

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SUMMARY

The discussion focuses on devising experiments in cell biology to confirm three specific statements regarding protein activity, particularly in the context of positive and negative regulation. Participants suggest utilizing techniques such as DNAase II sensitivity assays, Electrophoretic Mobility Shift Assays (EMSA), and DNA footprinting to investigate these regulatory mechanisms. The emphasis is on designing experiments that effectively measure protein interactions and their regulatory effects on DNA.

PREREQUISITES
  • Understanding of DNAase II sensitivity assays
  • Familiarity with Electrophoretic Mobility Shift Assays (EMSA)
  • Knowledge of DNA footprinting techniques
  • Basic concepts of protein regulation mechanisms
NEXT STEPS
  • Research the methodology of DNAase II sensitivity assays
  • Learn about the principles and applications of EMSA
  • Explore advanced techniques in DNA footprinting
  • Investigate the mechanisms of positive and negative protein regulation
USEFUL FOR

Cell biologists, molecular biologists, and researchers involved in experimental design related to protein activity and regulation will benefit from this discussion.

niravana21
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Cell Biology -- Devising Experiments

Homework Statement



I've included a picture of the problem to avoid confusion

2. The attempt at a solution
I need to devise an experiment that would confirm the 3 statements. By experiements, it means something along the line of DNAase II sensitivity assay, EMSA, DNA footprinting etc

What kind of experiments can determine protien activity? Specially, determining positive and negative regulation?
 

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niravana21 said:
I've included a picture of the problem to avoid confusion

Nobody but you can see the picture. And you can see it because you have it in your cache and/or you are logged into your gmail account.
 


it should be attached now
 

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