Chevron plot of protein folding kinetics with denaturant

[biophyscis]

Homework Statement

Folding can be described as transition between the native and unfolded state. In increasing concentrations of denaturant, a fold disintegrates with a rate

lnKU = ln KU H20 - m ku [denat]

when the concentration of denaturant is above that needed to unfold 50 % of protein. Conversely folding takes place with rate

lnKf = ln Kf H2O - m ku [denat]

when the concentration of denaturant is below that needed to unfold 50 % of protein.
Write an equation for the observed rate constant ln kobs and plot it (Chevron plot) as a function of denaturant concentration.
How do you determine from the figure kfH2O?
How will the Chevron plot change when a mutation will affect the transition state?

Homework Equations

The Attempt at a Solution

 

[epenguin]

Homework Statement

Folding can be described as transition between the native and unfolded state. In increasing concentrations of denaturant, a fold disintegrates with a rate

lnKU = ln KU H20 - m ku [denat]

when the concentration of denaturant is above that needed to unfold 50 % of protein. Conversely folding takes place with rate

lnKf = ln Kf H2O - m ku [denat]

when the concentration of denaturant is below that needed to unfold 50 % of protein.
Write an equation for the observed rate constant ln kobs and plot it (Chevron plot) as a function of denaturant concentration.
How do you determine from the figure kfH2O?
How will the Chevron plot change when a mutation will affect the transition state?

Homework Equations

The Attempt at a Solution

What looks like an identical post dated Jun 2 was answered on Jun 3