Converting cDNA to Protein Sequence: Methods and Recommendations

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Discussion Overview

The discussion centers on methods and recommendations for converting cDNA to protein sequences, focusing on protocols and tools available for this process. Participants share their experiences and seek advice on practical approaches.

Discussion Character

  • Exploratory, Technical explanation, Homework-related

Main Points Raised

  • One participant inquires about recommendations for kits and protocols for converting cDNA to protein sequences.
  • Another participant seeks clarification on whether the initial goal is to derive the protein sequence from a given cDNA sequence.
  • A participant confirms they have the cDNA and expresses familiarity with obtaining it via PCR, but is unsure about the subsequent steps to obtain the protein sequence.
  • One suggestion involves sending the cDNA to a sequencing facility and using an online translation tool to derive the protein sequence from the obtained sequence.

Areas of Agreement / Disagreement

The discussion does not reach a consensus on specific methods or kits, and participants express varying levels of familiarity with the process.

Contextual Notes

Some participants may have assumptions about the availability of resources or the ease of the process, which are not explicitly stated. The discussion does not clarify the specific requirements for sequencing or the choice of translation tools.

Who May Find This Useful

Researchers or students interested in molecular biology techniques, particularly those working with cDNA and protein sequencing.

gravenewworld
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Has anyone here done this before? If so, kit recommendations and protocol? Any help would be awesome. Thanks!
 
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What exactly do you want to do? Do you have a cDNA sequence and you want to figure out the protein sequence that it codes for?
 
Yes exactly. Getting the cDNA is easy, I have experience with that for PCR, but instead of doing PCR, I was wondering how you can use your cDNA to get the protein sequence. My PI said it should be easily doable. I'm not sure how though.
 
Once you have the cDNA sequence, you just need to sequence it (send a sample of the cDNA along with the appropriate primers to a DNA sequencing facility/company). You can the just read the protein sequence from the sequence you get (for example, using an online translation tool such as http://www.bioinformatics.org/sms2/translate.html

Let me know if you want more detail on any of these steps.
 

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