- #1
hagopbul
- 397
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Making of dna ?
who it,s done
who it,s done
Creating Artificial DNA: Fact or Fiction?
- Thread starter hagopbul
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Discussion Overview
The discussion revolves around the creation of artificial DNA, exploring both natural processes of DNA replication and laboratory methods for synthesizing DNA. Participants examine the complexities of DNA structure, replication, and the feasibility of creating DNA in a lab setting.
Discussion Character
- Exploratory, Technical explanation, Debate/contested
Main Points Raised
- One participant describes the basic process of DNA replication, emphasizing the role of base pairs and the splitting of DNA strands.
- Another participant asserts that DNA cannot be created in the laboratory without a template, suggesting that it must be extracted from cells.
- A different participant mentions that they can "make" DNA in the lab using PCR, but clarifies that this requires a template and cannot produce a whole genome.
- Some participants discuss the possibility of creating small DNA strands based on known sequences, with one mentioning a company that re-engineers genes for expression in non-native species.
- There is a reference to a claim about artificial DNA being created at the University of Texas, prompting questions about its validity.
- One participant indicates that the lab is attempting to create an "artificial" genome using an oligo-synthesizer, distinguishing this method from PCR.
Areas of Agreement / Disagreement
Participants express differing views on the feasibility of creating DNA in the laboratory, with some asserting it is impossible without a template, while others suggest that small strands can be synthesized. The discussion remains unresolved regarding the claim about artificial DNA created at the University of Texas.
Contextual Notes
Participants reference various methods and processes related to DNA synthesis and replication, but there are limitations in the discussion regarding the specifics of these methods and the definitions of "artificial DNA." Unresolved assumptions about the capabilities of current laboratory techniques are present.
- #2
Schrodinger's Dog
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Well do you want the short answer or to take a 3 month course in genetics.
If you just mean how does DNA replicate itself, it splits apart into two chains of DNA, this is fairly easy to do as there are weak bonds between the base pairs.
The base pairs
Adanine
Gaunine
Thymine
Cytosine
Click to Enlarge.^
May have seen a code like this before to express say a gene
AGCTCTCCCTAAGGG... etcBecause Adanine only pairs with Thymine and Gaunine with Cytosine. The free base pairs in the Nucleus will pair with these to form two strands and two copies, one of which then breaks off.
AGCCT
¦¦ ¦¦¦
TCGGA
I haven't gone into the enzymes and how they achieve this or any great detail, to be frank it's an complicated process, but simply speaking this is how DNA is replicated. As for how it's made, or how a cell splits into two complete cells: mitosis, or what it's made from, a Google search will help there.
http://en.wikipedia.org/wiki/Mitosis"
http://en.wikipedia.org/wiki/DNA" Once these chains have been replicated from the DNA nucleus, single strands of DNA can then be used to encode proteins in various areas, I could explain how they do this by using tRNA and mRNA (transposeRNA and messenger RNA) but this is another long winded process involving 3 base pair codons for each protein.
http://web.jjay.cuny.edu/~acarpi/NSC/12-dna.htm
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Codons.html
Here's a list of all the codons and there respective polypeptides(the building blocks of proteins)
You may want to be a bit more specific, this is a large area and a complicated one.
If you just mean how does DNA replicate itself, it splits apart into two chains of DNA, this is fairly easy to do as there are weak bonds between the base pairs.
The base pairs
Adanine
Gaunine
Thymine
Cytosine
Click to Enlarge.^
May have seen a code like this before to express say a gene
AGCTCTCCCTAAGGG... etcBecause Adanine only pairs with Thymine and Gaunine with Cytosine. The free base pairs in the Nucleus will pair with these to form two strands and two copies, one of which then breaks off.
AGCCT
¦¦ ¦¦¦
TCGGA
I haven't gone into the enzymes and how they achieve this or any great detail, to be frank it's an complicated process, but simply speaking this is how DNA is replicated. As for how it's made, or how a cell splits into two complete cells: mitosis, or what it's made from, a Google search will help there.
http://en.wikipedia.org/wiki/Mitosis"
http://en.wikipedia.org/wiki/DNA" Once these chains have been replicated from the DNA nucleus, single strands of DNA can then be used to encode proteins in various areas, I could explain how they do this by using tRNA and mRNA (transposeRNA and messenger RNA) but this is another long winded process involving 3 base pair codons for each protein.
http://web.jjay.cuny.edu/~acarpi/NSC/12-dna.htm
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Codons.html
Here's a list of all the codons and there respective polypeptides(the building blocks of proteins)
You may want to be a bit more specific, this is a large area and a complicated one.
Last edited by a moderator:
- #3
HallsofIvy
Science Advisor
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If you are asking "How is DNA made in the laboratory?", it isn't. DNA has to be taken from a cell.
- #4
Rade
This site:HallsofIvy said:
http://www.downtoearth.org.in/full6.asp?foldername=20000228&filename=news&sec_id=4&sid=1
claims artificial DNA created at U of Texas ?? any truth to this ??
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- #5
iansmith
Staff Emeritus
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I "make" DNA everyday in the lab using PCR but I need a template to do this. I just can't make a whole genome.
It is possible to create small DNA strand without any template but this is often based on know sequences. However, there a company that re-engineers genes that will be introduced and expressed at higher level in a non-native species. The company changes the sequence of the gene and basically creates a new version.
As fas as I know, it is real and the lab is try to create an "artificial" genome using an "DNA" oligo-synthesizer rather than PCR.
It is possible to create small DNA strand without any template but this is often based on know sequences. However, there a company that re-engineers genes that will be introduced and expressed at higher level in a non-native species. The company changes the sequence of the gene and basically creates a new version.
Rade said:
As fas as I know, it is real and the lab is try to create an "artificial" genome using an "DNA" oligo-synthesizer rather than PCR.
Last edited by a moderator:
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