Creating Artificial DNA: Fact or Fiction?

[hagopbul]

Making of dna ?
who it,s done

 

[Schrodinger's Dog]

Well do you want the short answer or to take a 3 month course in genetics.

If you just mean how does DNA replicate itself, it splits apart into two chains of DNA, this is fairly easy to do as there are weak bonds between the base pairs.

The base pairs

Adanine
Gaunine
Thymine
Cytosine

Click to Enlarge.^

May have seen a code like this before to express say a gene

AGCTCTCCCTAAGGG... etcBecause Adanine only pairs with Thymine and Gaunine with Cytosine. The free base pairs in the Nucleus will pair with these to form two strands and two copies, one of which then breaks off.

AGCCT
¦¦ ¦¦¦
TCGGA

I haven't gone into the enzymes and how they achieve this or any great detail, to be frank it's an complicated process, but simply speaking this is how DNA is replicated. As for how it's made, or how a cell splits into two complete cells: mitosis, or what it's made from, a Google search will help there.

http://en.wikipedia.org/wiki/Mitosis"

http://en.wikipedia.org/wiki/DNA" Once these chains have been replicated from the DNA nucleus, single strands of DNA can then be used to encode proteins in various areas, I could explain how they do this by using tRNA and mRNA (transposeRNA and messenger RNA) but this is another long winded process involving 3 base pair codons for each protein.

http://web.jjay.cuny.edu/~acarpi/NSC/12-dna.htm

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Codons.html

Here's a list of all the codons and there respective polypeptides(the building blocks of proteins)

You may want to be a bit more specific, this is a large area and a complicated one.

 

[HallsofIvy]

If you are asking "How is DNA made in the laboratory?", it isn't. DNA has to be taken from a cell.

 

[Rade]

If you are asking "How is DNA made in the laboratory?", it isn't. DNA has to be taken from a cell.

This site:
http://www.downtoearth.org.in/full6.asp?foldername=20000228&filename=news&sec_id=4&sid=1

claims artificial DNA created at U of Texas ?? any truth to this ??

 

[iansmith]

I "make" DNA everyday in the lab using PCR but I need a template to do this. I just can't make a whole genome.

It is possible to create small DNA strand without any template but this is often based on know sequences. However, there a company that re-engineers genes that will be introduced and expressed at higher level in a non-native species. The company changes the sequence of the gene and basically creates a new version.

This site:
http://www.downtoearth.org.in/full6.asp?foldername=20000228&filename=news&sec_id=4&sid=1

claims artificial DNA created at U of Texas ?? any truth to this ??

As fas as I know, it is real and the lab is try to create an "artificial" genome using an "DNA" oligo-synthesizer rather than PCR.