Do Ribosomes, tRNA, and mRNA Interact to Synthesize Proteins?

[quietrain]

1) am i right to say ribosomes are the sites where tRNA and mRNA meet to produce proteins? by the way, is it proteins or amino acids?

2) if i want to form 5 amino acids, does it mean i need 5 tRNAs? do i need to count in the stop codon or release factor? but since stop codon is mRNA, and release factor is not tRNA, does it mean 5 amino acids means i need 5 tRNAs?

3) if i synthesize 5 amino acids, does it mean i get 5 water molecules? since peptide bonds are formed by losing 1 water molecule. so 5 amino acids means 5 water molecules? do i need to take into account anything else?

thank you very much!

 

[Ygggdrasil]

1) am i right to say ribosomes are the sites where tRNA and mRNA meet to produce proteins? by the way, is it proteins or amino acids?

Yes, the binding of mRNAs to tRNAs occurs on the ribosome. This process produces protein. Proteins are made up of amino acids connected in a linear chain.

2) if i want to form 5 amino acids, does it mean i need 5 tRNAs? do i need to count in the stop codon or release factor? but since stop codon is mRNA, and release factor is not tRNA, does it mean 5 amino acids means i need 5 tRNAs?

Yes, a protein of 5 amino acids would require 5 tRNAs, one for each amino acid.

3) if i synthesize 5 amino acids, does it mean i get 5 water molecules? since peptide bonds are formed by losing 1 water molecule. so 5 amino acids means 5 water molecules? do i need to take into account anything else?

It's actually four, not five (since there are four reactions going on, a reaction linking amino acids 1 and 2, linking 2 and 3, linking 3 and 4 and linking 4 and 5). I should also note that releasing the protein from the ribosome requires a water molecule.

 

[tkjtkj]

Re: "It's actually four, not five (since there are four reactions going on, a reaction linking amino acids 1 and 2, linking 2 and 3, linking 3 and 4 and linking 4 and 5). I should also note that releasing the protein from the ribosome requires a water molecule."

So, net gain is 3 H2O's
;)

 

[jackmell]

2) if i want to form 5 amino acids, does it mean i need 5 tRNAs?

No, and I'm going against Ygg up there. You said you want to "form" five amino acids. Well, I don't think tRNA is involved with "forming" amino acids. In general amino acids come from our diet although I think the body can synthesize many of them during intermediate metabolism but tRNA is not involved in that synthesis I think. Just check alright. Google how the body synthesizes amino acids. Be good for you right?

 

[mishrashubham]

No, and I'm going against Ygg up there. You said you want to "form" five amino acids.

Well I agree that the OP should not use the word 'form' or 'synthesize' in such context. But he is definitely talking about translation over here (he mentions tRNA, mRNA, stop codon and release factor).

 

[jackmell]

Need to be precise in science. Oh, just put a few drops of hydrochloric acid in the eyes . . . wait, sorry, I meant boric acid. What else? Just one little slip-up and bam-o. Dead dog. Oh, I meant one mole, not two. That dose-dependent thing too: one pill is therapeudic, two pills though kill. I know he's new. Better learn now though. Just trying to help.

 

[mishrashubham]

Need to be precise in science.

Totally Agree.