Folding Kinetics: Equation & Chevron Plot

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    Folding Kinetics
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SUMMARY

The discussion focuses on the kinetics of protein folding and unfolding, specifically using the equations for the rate constants lnKU and lnKf in relation to denaturant concentration. The observed rate constant ln kobs is derived from these equations, which are critical for constructing a Chevron plot that illustrates the folding and unfolding processes. Additionally, the impact of mutations on the transition state and its representation in the Chevron plot is explored, providing insights into protein stability and folding dynamics.

PREREQUISITES
  • Understanding of protein folding and unfolding kinetics
  • Familiarity with the concepts of denaturants and their effects on protein stability
  • Knowledge of Chevron plots and their significance in biochemistry
  • Basic proficiency in mathematical modeling of biochemical reactions
NEXT STEPS
  • Study the derivation of the observed rate constant ln kobs in protein folding
  • Learn how to construct and interpret Chevron plots in protein chemistry
  • Investigate the effects of specific mutations on protein folding kinetics
  • Explore advanced topics in protein stability and denaturation mechanisms
USEFUL FOR

Biochemists, molecular biologists, and researchers studying protein dynamics, as well as students seeking to understand the principles of protein folding and the effects of mutations on stability.

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Homework Statement


Folding can be described as transition between the native and unfolded state. In increasing concentrations of denaturant, a fold disintegrates with a rate

lnKU = ln KU H20 - m ku [denat]

when the concentration of denaturant is above that needed to unfold 50 % of protein. Conversely folding takes place with rate

lnKf = ln Kf H2O - m ku [denat]

when the concentration of denaturant is below that needed to unfold 50 % of protein.
Write an equation for the observed rate constant ln kobs and plot it (Chevron plot) as a function of denaturant concentration.
How do you determine from the figure kfH2O?
How will the Chevron plot change when a mutation will affect the transition state?


Homework Equations





The Attempt at a Solution

 
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biophysics said:

Homework Statement


Folding can be described as transition between the native and unfolded state. In increasing concentrations of denaturant, a fold disintegrates with a rate

lnKU = ln KU H20 - m ku [denat]

when the concentration of denaturant is above that needed to unfold 50 % of protein. Conversely folding takes place with rate

lnKf = ln Kf H2O - m ku [denat]

when the concentration of denaturant is below that needed to unfold 50 % of protein.
Write an equation for the observed rate constant ln kobs and plot it (Chevron plot) as a function of denaturant concentration.
How do you determine from the figure kfH2O?
How will the Chevron plot change when a mutation will affect the transition state?


Homework Equations





The Attempt at a Solution


What looks like an identical post dated Jun 2 was answered on Jun 3
 

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