Biology Genetics Chromosomal Inversion problem

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    Genetics Inversion
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To solve the chromosomal inversion problem, the sequence between nucleotides 4 and 12 must be inverted while maintaining the correct orientation for double-stranded DNA. The original sequence is 5'-TCTCGCATGGTAGGT-3', and the segment to be inverted is 5'-GCATGGT-3'. After inversion, the correct rejoining should result in the sequence 5'-TCTCGTGGTACGAGGT-3'. The confusion arises from the need to ensure that the ends of the strands are properly oriented, as DNA strands must be antiparallel. Clarification on the proper method to rejoin the inverted segment is essential for completing the assignment correctly.
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Homework Statement


Rewrite following sequence as double stranded molecule, with an inversion of the sequence between nucleotide 4 and 12.

5'-TCTCGCATGGTAGGT-3'

Homework Equations


none.

The Attempt at a Solution


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I've read in the textbook that you take the section and rotate it 180 degrees, then reinsert it. However I think that I'm not understanding this properly because when I do this, I get a 5' end that needs to be rejoined with another 5' end, and likewise with the 3' end:

Original: 5'-TCTCGCATGGTAGGT-3'
Taking out sequence which needs to be inverted: 5'-GCATGGT-3'
Invert: 3'-TGGTACG-5'
Inverted sequence:5'-TCTC-3' (3'-TGGTACG-5') 5'-AGGT-3' ...so I don't see how I can rejoin the inverted segment.

I feel like I'm missing something obvious, if someone could help me out with this it would be great.
 
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Remember that DNA is double stranded. When you rotate the DNA 180° will the same strand of DNA be on top?
 
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