Genetics Chromosomal Inversion problem

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SUMMARY

The discussion focuses on the problem of performing a chromosomal inversion on a DNA sequence, specifically the sequence 5'-TCTCGCATGGTAGGT-3'. The user attempts to invert the segment between nucleotides 4 and 12, resulting in confusion regarding the proper orientation of the strands. The correct approach involves extracting the segment 5'-GCATGGT-3', inverting it to 3'-TGGTACG-5', and then rejoining it with the remaining sequences, ensuring that the 5' and 3' ends are correctly aligned. The user seeks clarification on maintaining the double-stranded nature of DNA during this process.

PREREQUISITES
  • Understanding of DNA structure and terminology, including 5' and 3' ends.
  • Knowledge of chromosomal inversion and its implications in genetics.
  • Familiarity with nucleotide sequences and their representation.
  • Basic grasp of molecular biology concepts related to DNA manipulation.
NEXT STEPS
  • Study the mechanics of DNA inversion and its biological significance.
  • Learn about DNA strand orientation and the implications for molecular cloning techniques.
  • Explore tools for visualizing DNA sequences and manipulations, such as SnapGene.
  • Research the effects of chromosomal inversions on gene expression and genetic variation.
USEFUL FOR

Students in genetics, molecular biologists, and educators looking to deepen their understanding of DNA structure and manipulation techniques, particularly in the context of chromosomal inversions.

David23454
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Homework Statement


Rewrite following sequence as double stranded molecule, with an inversion of the sequence between nucleotide 4 and 12.

5'-TCTCGCATGGTAGGT-3'

Homework Equations


none.

The Attempt at a Solution


[/B]
I've read in the textbook that you take the section and rotate it 180 degrees, then reinsert it. However I think that I'm not understanding this properly because when I do this, I get a 5' end that needs to be rejoined with another 5' end, and likewise with the 3' end:

Original: 5'-TCTCGCATGGTAGGT-3'
Taking out sequence which needs to be inverted: 5'-GCATGGT-3'
Invert: 3'-TGGTACG-5'
Inverted sequence:5'-TCTC-3' (3'-TGGTACG-5') 5'-AGGT-3' ...so I don't see how I can rejoin the inverted segment.

I feel like I'm missing something obvious, if someone could help me out with this it would be great.
 
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Remember that DNA is double stranded. When you rotate the DNA 180° will the same strand of DNA be on top?
 
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