Genetics problem-Interrupted mating

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SUMMARY

The discussion centers on the genetics problem regarding interrupted mating involving three Hfr strains derived from the same F+ bacteria. The participants analyze gene alignment discrepancies between the strains, noting that the first and second sets do not match completely, despite expectations. The correct answer to the posed question is confirmed as option (a), with R and S being correct, while the inclusion of P requires further clarification. The explanation provided suggests that Hfr2 is an e- strain, which complicates the observation of gene transfer.

PREREQUISITES
  • Understanding of Hfr strains and F+ bacteria in bacterial genetics
  • Knowledge of gene alignment techniques and discrepancies
  • Familiarity with interrupted mating and gene transfer mechanisms
  • Basic concepts of bacterial conjugation and genetic mapping
NEXT STEPS
  • Research the mechanisms of gene transfer in Hfr strains
  • Study the implications of e- strains in genetic experiments
  • Learn about bacterial conjugation and its role in genetic variation
  • Explore methods for aligning genetic sequences and interpreting discrepancies
USEFUL FOR

Genetics students, microbiologists, and researchers involved in bacterial genetics and gene transfer studies will benefit from this discussion.

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Homework Statement
The following table shows mapping data from 3 interrupted mating experiments using three different Hfr strains and F- strain:
Relevant Equations
Please check the picture below.
244058


My Attempt:
It appears to me that the three Hfr strains are derived from the same F+ bacteria. This info is absent in the question.

Now on aligning all three sets of genes, the first and second set doesn't match completely.
244059

I do not understand why it doesn't as they are supposed to be generated from the same F+ bacteria.

The correct answer for this Question is (a).
R and S are correct so option (a) should be correct, but how's P too correct.
 
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SanjuktaGhosh said:
My Attempt:
It appears to me that the three Hfr strains are derived from the same F+ bacteria. This info is absent in the question.

This is not necessarily true and an explanation of the results require some differences between the Hfr strains.

Now on aligning all three sets of genes, the first and second set doesn't match completely.
View attachment 244059
I do not understand why it doesn't as they are supposed to be generated from the same F+ bacteria.

One explanation for the results is that Hfr2 is an e- strain, so transfer of e cannot be observed.
 

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