How can I improve my HPLC method for beta-alanine?

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The discussion centers on challenges faced in developing an HPLC method for analyzing beta-alanine using an Agilent system. The user reports difficulties achieving a satisfactory peak with their current method, which employs a gradient mobile phase consisting of 0.1% formic acid in both water and acetonitrile, utilizing a 250 mm C18 column at a flow rate of 1.0 µL. The detection wavelength is set at 214 nm with a 10 µL injection volume at ambient temperature. The gradient profile includes specific ratios of mobile phases over time, but the user expresses uncertainty about the effectiveness of their current setup. Suggestions and troubleshooting advice from other forum members are sought to improve peak resolution and overall method performance.

May 17, 2023

#1

Rayyana123

I am working on HPLC method Beta alanine and unable to get a good enough peak. I'm using an agilent system HPLC ,gradient mobile phase(a: 0.1% formic Acid in Ro water,0.1% Formic acid in ACN) 250 mm C18 4/6x 250 mm column Flow rate 1.0 ul Detector: 214 nm 10 ul injected temp:ambient
Gradient below min A: B: 0 95 5 2 75 25 10 50 50 12 5 95 15 5 95 Im unsure on what I'm not doing correctly! Any help will be appreciated!

Reference: https://www.physicsforums.com/forums/chemistry.83/