How can I improve my HPLC method for beta-alanine?

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SUMMARY

The discussion focuses on improving the HPLC method for beta-alanine using an Agilent HPLC system. The user employs a gradient mobile phase consisting of 0.1% formic acid in both RO water and acetonitrile, utilizing a 250 mm C18 column with a flow rate of 1.0 µL. The detector is set to 214 nm with a 10 µL injection at ambient temperature. The user seeks assistance to achieve a better peak resolution in their chromatographic analysis.

PREREQUISITES
  • Understanding of HPLC principles and operation
  • Familiarity with gradient elution techniques
  • Knowledge of C18 column characteristics
  • Experience with UV detection at 214 nm
NEXT STEPS
  • Optimize the gradient profile for better peak resolution
  • Investigate the effects of varying the flow rate on peak shape
  • Explore alternative mobile phase compositions for beta-alanine
  • Learn about column temperature effects on HPLC performance
USEFUL FOR

Chemists, analytical laboratory technicians, and researchers involved in HPLC method development and optimization for amino acid analysis.

Rayyana123
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I am working on HPLC method Beta alanine and unable to get a good enough peak. I'm using an agilent system HPLC ,gradient mobile phase(a: 0.1% formic Acid in Ro water,0.1% Formic acid in ACN) 250 mm C18 4/6x 250 mm column Flow rate 1.0 ul Detector: 214 nm 10 ul injected temp:ambient
Gradient below min A: B: 0 95 5 2 75 25 10 50 50 12 5 95 15 5 95 Im unsure on what I'm not doing correctly! Any help will be appreciated!

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