How can I improve my HPLC method for beta-alanine?

  • Thread starter Thread starter Rayyana123
  • Start date Start date
  • Tags Tags
    Method
AI Thread Summary
The discussion centers on challenges faced in developing an HPLC method for analyzing beta-alanine using an Agilent system. The user reports difficulties achieving a satisfactory peak with their current method, which employs a gradient mobile phase consisting of 0.1% formic acid in both water and acetonitrile, utilizing a 250 mm C18 column at a flow rate of 1.0 µL. The detection wavelength is set at 214 nm with a 10 µL injection volume at ambient temperature. The gradient profile includes specific ratios of mobile phases over time, but the user expresses uncertainty about the effectiveness of their current setup. Suggestions and troubleshooting advice from other forum members are sought to improve peak resolution and overall method performance.
Rayyana123
Messages
1
Reaction score
0
I am working on HPLC method Beta alanine and unable to get a good enough peak. I'm using an agilent system HPLC ,gradient mobile phase(a: 0.1% formic Acid in Ro water,0.1% Formic acid in ACN) 250 mm C18 4/6x 250 mm column Flow rate 1.0 ul Detector: 214 nm 10 ul injected temp:ambient
Gradient below min A: B: 0 95 5 2 75 25 10 50 50 12 5 95 15 5 95 Im unsure on what I'm not doing correctly! Any help will be appreciated!

Reference: https://www.physicsforums.com/forums/chemistry.83/
 

Thread 'Humidity of a saturated NaCl solution with anticaking additive'

I want to test a humidity sensor with one or more saturated salt solutions. The table salt that I have on hand contains one of two anticaking agents, calcium silicate or sodium aluminosilicate. Will the presence of either of these additives (or iodine for that matter) significantly affect the equilibrium humidity? I searched and all the how-to-do-it guides did not address this question. One research paper I found reported that at 1.5% w/w calcium silicate increased the deliquescent point by...
View full post »

Thread 'Why does having 8 valence electrons make an element inert?'

I was introduced to the Octet Rule recently and make me wonder, why does 8 valence electrons or a full p orbital always make an element inert? What is so special with a full p orbital? Like take Calcium for an example, its outer orbital is filled but its only the s orbital thats filled so its still reactive not so much as the Alkaline metals but still pretty reactive. Can someone explain it to me? Thanks!!
View full post »

Thread 'Resists for etching Aluminium with NaOH'

I'm trying to find a cheap DIY method to etch holes of various shapes through 0.3mm Aluminium sheet using 5-10% Sodium Hydroxide. The idea is to apply a resist to the Aluminium then selectively ablate it off using a diode laser cutter and then dissolve away the Aluminium using Sodium Hydroxide. By cheap I mean resists costing say £20 in small quantities. The Internet has suggested various resists to try including... Enamel paint (only survived seconds in the NaOH!) Acrylic paint (only...
View full post »

Similar threads

B How can I linearize my (negative slope) quadratic graph?
Replies
16
Views
5K
How Can Plasma Be Created and Ignited in Toroidal Fusion Reactors?
Replies
4
Views
4K
How can I avoid large exponents in calculations with small answers?
Replies
5
Views
3K

Hot Threads

Recent Insights

Back
Top