How Do You Calculate Gain in Sinusoidal Signals?

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how does one calculate a gain in signal e.g sine wave. do we take peak-to-peak (or peak) of the output divided by peak-to-peak(or peak) of the input?
 
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The ratio of the peak-to-peak voltages and RMS will be the same (both are related by the same factor of 1/sqrt2 if the signal is sinusoidal).
Gain is defined as 20dB*log(Vout/Vin)
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