We know that Vmax depends on enzyme concentration since Vmax = k2[E] However, what I have trouble grasp is that why Km does not depend on enzyme concentration if Km is the substrate concentration where V = 1/2 Vmax. If you increase Vmax, shouldn't Km increase as well since it is dependent on it? I asked my biochem prof today and he gave some explaination about substrate concentration which I didn't catch. I am hoping someone can clarify it here. thanks!