Made biodiesel from pumpkin oil

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i have made biodiesel from pumpkin oil and samples of B10 B20,...,B100. i want to get optical properties of these samples.what should be these poperties?i want to determine refractive index and also want to use raman spectroscopy.can i do this??can i use raman?
 
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Of course you may characterize it by Raman. I don't believe you can determine the RI with a Raman instrument, though.