On the Importance of Positive Controls

  • #1
Ygggdrasil
Science Advisor
Insights Author
Gold Member
3,283
3,412
Found this abstract while browsing through some papers, and thought it was quite humorous:
Yeast-based biosynthesis of medicinal compounds traditionally derived from plant materials is improving. Both concerns and hopes exist for the possibility that individual small volume batch fermentations could provide distributed and independent access to a diversity of compounds some of which are now abused, illegal, or unavailable to many who need for genuine medical purposes. However, there are differences between industrial bioreactors and ‘home-brew’ fermentation. We used engineered yeast that make thebaine, a morphinan opiate, to quantify if differences in fermentation conditions impact biosynthesis yields. We used yeast that make an English ale as a positive fermentation control. We observed no production of thebaine and miniscule amounts of reticuline, an upstream biosynthetic intermediate, in home-brew fermentations; the positive control was palatable. We suggest that additional technical challenges, some of which are unknown and likely unrelated to optimized production in large-volume bioreactors, would need to be addressed for engineered yeast to ever realize home-brew biosynthesis of medicinal opiates at meaningful yields.

Endy, Galanie & Smolke. 2015 Complete absence of thebaine biosynthesis under home-brew fermentation conditions. bioRxiv doi:10.1101/024299

Full text freely available at http://biorxiv.org/content/early/2015/08/13/024299
 
  • Like
Likes Drakkith and mfb

Answers and Replies

  • #2
35,340
11,650
Alternative introduction: "We found a reason to make beer at home using science grants".
 
  • #3
jim hardy
Science Advisor
Gold Member
Dearly Missed
9,839
4,882
We observed no production of thebaine and miniscule amounts of reticuline, an upstream biosynthetic intermediate, in home-brew fermentations;
So - no 'opiate for the masses' ?
 
  • #4
Ygggdrasil
Science Advisor
Insights Author
Gold Member
3,283
3,412
So - no 'opiate for the masses' ?
Not at the moment, at least.
 

Related Threads on On the Importance of Positive Controls

  • Last Post
Replies
3
Views
2K
  • Last Post
Replies
4
Views
10K
Z
Replies
47
Views
5K
  • Last Post
Replies
5
Views
3K
Replies
4
Views
457
  • Last Post
Replies
5
Views
3K
  • Last Post
Replies
2
Views
2K
  • Last Post
Replies
5
Views
2K
  • Last Post
3
Replies
60
Views
5K
Top