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Photoactivated localization microscopy (PALM)

  1. May 31, 2014 #1
    I have been reading up on PALM works and am still confused about a few things. In particular, (1) how can you only excite a few molecules at a time and then only photobleach those molecules? Won't other molecules also automatically be excited and eventually photobleached? (2) When you fit the point-spread function to a Gaussian function, what exactly are you doing? Isn't it basically just taking the weighted average of all the intensities at each pixel? Thanks!
     
  2. jcsd
  3. May 31, 2014 #2

    UltrafastPED

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