- #1
vande060
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Homework Statement
prepare 40mM TES buffer pH 7.4
prepare 100mls of it
mw TES = 229.25
pka = 7.5
Homework Equations
ph = pka +log(A-/HA)
The Attempt at a Solution
My problem is that I don't know what the MW of the acid or base is for this buffer. Is the given MW the acid, and I subract a proton weight for the base or something? I can do the calculation though:
7.4 = 7.5 +log(A-/HA)
-.1 = log(A-/HA)
A-/HA = .79
56% base
44% acid
.56* 40mM * MW of base *.1L = moles of base
.44*40mM *MW of acid *.1L = moles of acid
So how should I figure out the MW of acid and base?