Question about spectrophotometry and resveratrol

[jackscholar]

Homework Statement

I need to determine the concentration of resveratrol in my sample of wine. How would i do this using spectrophotometry? I know it has an absorbance of 306nm in an ethonal solution, but I've only good wine. Do I create an absorbance graph of known resveratrol concentrations in ethanol at306nm and create a graph, then measure the absorbance of the wine and compare it to the graph?

 

[hyddro]

yes, I would definitely make a graph with different concentrations of resveratrol in ethanol, this will give you a good relantion between concentration and absorbance from there you can calculate the concentration of it. Also I would do 3 trials for each known concentration, just to make sure you are on the right standard diviation.

 

[Ygggdrasil]

It's worth noting that wine is a complex mixture and could contain other compounds that could absorb at 306 nm and interfere with your measurements.

 

[chemisttree]

Use 12% ethanol in water for your stock solutions rather than plain ethanol.