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Question about spectrophotometry and resveratrol

  1. Mar 27, 2013 #1
    1. The problem statement, all variables and given/known data
    I need to determine the concentration of resveratrol in my sample of wine. How would i do this using spectrophotometry? I know it has an absorbance of 306nm in an ethonal solution, but i've only good wine. Do I create an absorbance graph of known resveratrol concentrations in ethanol at306nm and create a graph, then measure the absorbance of the wine and compare it to the graph?
     
  2. jcsd
  3. Mar 28, 2013 #2
    yes, I would definitely make a graph with different concentrations of resveratrol in ethanol, this will give you a good relantion between concentration and absorbance from there you can calculate the concentration of it. Also I would do 3 trials for each known concentration, just to make sure you are on the right standard diviation.
     
  4. Mar 29, 2013 #3

    Ygggdrasil

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    It's worth noting that wine is a complex mixture and could contain other compounds that could absorb at 306 nm and interfere with your measurements.
     
  5. Mar 29, 2013 #4

    chemisttree

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    Use 12% ethanol in water for your stock solutions rather than plain ethanol.
     
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