Raman spectroscopy amide I processing

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SUMMARY

This discussion focuses on the analysis of Raman spectra, specifically the processing of the amide I band in proteins. Key tools mentioned include Peakfit and Origin, with techniques such as Savitsky-Golay smoothing and amide I deconvolution being critical for quantifying secondary structures. The objective is to monitor changes in secondary structure within complex coacervates formed by proteins and polysaccharides. Participants seek guidance on the specific steps involved in deconvoluting the amide I band using these software tools.

PREREQUISITES
  • Understanding of Raman spectroscopy principles
  • Familiarity with Peakfit software
  • Knowledge of Origin software for data analysis
  • Experience with Savitsky-Golay smoothing technique
NEXT STEPS
  • Research the amide I band characteristics in Raman spectroscopy
  • Learn the step-by-step process of amide I deconvolution using Peakfit
  • Explore the application of Savitsky-Golay smoothing in spectral analysis
  • Investigate secondary structure quantification methods in proteins
USEFUL FOR

Researchers in biochemistry, analytical chemists, and anyone involved in protein analysis using Raman spectroscopy will benefit from this discussion.

newlabguy
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Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.
 
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newlabguy said:
Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.

take some chemistry course or ask your boss to employ a chemist.
 

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