- #1

nobahar

- 497

- 2

## Homework Statement

I need to use the Lambert-Beer equation to plot optical density of a lactate solution.

## Homework Equations

[tex]A=\varepsilon\ell C[/tex]

(The path length is 1 cm)

## The Attempt at a Solution

This is one of my four samples (if you can show me where I'm going wrong with this then I can apply the correction to the others):

2.22 [tex]\mu[/tex]mol in 1.5ml, therefore there is 0.296 [tex]\mu[/tex]mol in 0.2ml

This info seems to match to the example graph I have.

But using the formula above, where [tex]\varepsilon[/tex]=6.22 for micromol/ml, I get:

6.22 x (0.296/0.2) micromol/ml = 9.2056

This doesn't match up with the graph I have! Any help appreciated! Please!

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