Calvin Cycle: Understanding Rubisco Structure & Mechanism

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Discussion Overview

The discussion revolves around the Calvin cycle, specifically focusing on the role of ribulose bisphosphate and the proposed formation of a 6-carbon sugar intermediate during the cycle. Participants explore the challenges of studying the structure and mechanism of the enzyme rubisco to clarify these points.

Discussion Character

  • Exploratory, Conceptual clarification, Debate/contested

Main Points Raised

  • One participant suggests that ribulose bisphosphate is converted into a 6-carbon sugar as a transient intermediate before forming phosphoglyceric acid.
  • Another participant questions the feasibility of synthesizing rubisco to determine its structure and mechanism, implying that it may be more complex than it appears.
  • A later reply indicates that while rubisco has been sequenced, understanding the specific intermediate remains elusive, raising questions about the difficulty of this research.
  • Further suggestions include using search engines to find definitions and information about the Calvin cycle, though one participant notes a lack of information regarding the 6-carbon intermediate.

Areas of Agreement / Disagreement

Participants express uncertainty about the existence and nature of the 6-carbon intermediate, with no consensus reached on the ease of synthesizing rubisco or the clarity of available information.

Contextual Notes

Limitations include the potential complexity of rubisco synthesis and the ambiguity surrounding the 6-carbon intermediate, which is not well-documented in available resources.

PhiJ
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I have been learning about the calvin cycle, and have been told that people think that ribulose bisphosphate is first converted into a 6 carbon sugar as a very short life intermediate, and then is converted to phosphoglyceric acid.
Why can't you just synthesise rubisco, work out its structure and mechanism of action etc. and then find if that is true. I'm sure its a lot harder than I'm thinking of it being, but how much?
 
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have you tried google?
 
I hadn't. Now I have though, I know that they have sequenced it, but still don't know about the intermediate, surely it would be easy to find... why is it so hard?
 
try

define: Calvin Cycle
 
I've tried it. Couldn't see anything about the 6 carbon intermediate though.
 

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