Using microscope for OPAQUE samples

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The discussion focuses on challenges faced when using an optical microscope to examine opaque samples due to inadequate lighting. The user describes difficulties in illuminating the sample as the microscope's lens arrangement blocks light from below, and attempts to use aluminum foil for reflection have failed. Suggestions include using a thinner or smaller sample to allow more light access and removing one eyepiece to shine light directly through the microscope. The conversation emphasizes the need for effective lighting solutions and possibly considering a different microscope for better results. Overall, achieving sufficient illumination is crucial for examining opaque samples effectively.
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I have an optical microscope and some opaque samples that I want to examine the surface of. I drew up a picture of how the microscope is arranged (attached). There is a series of lenses on a rotating drum that are located underneath the stage where glass slides are placed. The lenses usually come very close to the glass, almost touching it.
I'm having a lot of trouble lighting up the surface of the sample I need to look at. There needs to be quite a bit of light in order to get any decent image, and there is no space underneath to shine light from below. I have an optical fiber illuminator (brand Fiberlite) and it's still too big to snake through underneath. The lenses usually block all the light.
I tried to put aluminum foil on the bottom of the glass slide and use it to reflect the light fom above but that failed too, there was simply not enough light. I'm stuck right now and need some help.

Any ideas?
 

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Can you get a thinner sample? Or a smaller one in general, to get more light from the sides? The aluminium foil is a nice approach, but it works best if the sample is smaller than the height of the glass (+- some factor of 2).
 
You can use any opaque surface that is small in size and can reflect the light. It may be a metal.
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If the microscope is binocular, try removing one eyepiece and shining your light source down that.

This worked for me the other day to give me reflected light when I only had transmitted to work with.

Otherwise, I think you might need to find a better microscope.
 
Had my central air system checked when it sortta wasn't working. I guess I hadn't replaced the filter. Guy suggested I might want to get a UV filter accessory. He said it would "kill bugs and particulates". I know UV can kill the former, not sure how he thinks it's gonna murder the latter. Now I'm finding out there's more than one type of UV filter: one for the air flow and one for the coil. He was suggesting we might get one for the air flow, but now we'll have to change the bulb...

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