Using microscope for OPAQUE samples

  • Thread starter Thread starter refind
  • Start date Start date
  • Tags Tags
    Microscope
Click For Summary

Discussion Overview

The discussion centers around the challenges of using an optical microscope to examine opaque samples, particularly regarding lighting techniques and sample size. Participants explore various methods to enhance visibility of the sample surface without direct access to light from below.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested

Main Points Raised

  • One participant describes their setup with an optical microscope and the difficulties faced in illuminating opaque samples, noting the constraints of their current equipment.
  • Another participant suggests obtaining a thinner or smaller sample to allow more light to enter from the sides, indicating that the effectiveness of the aluminum foil method depends on the sample size relative to the glass slide.
  • A different participant proposes using any small opaque surface that can reflect light, mentioning metal as a potential option.
  • One participant recommends removing an eyepiece from a binocular microscope to shine light down that path, sharing a personal success with this method for achieving reflected light.
  • There is a suggestion that a better microscope may be necessary if current methods do not yield satisfactory results.

Areas of Agreement / Disagreement

Participants present multiple approaches to the problem, with no consensus on a single solution. Various suggestions are made, indicating differing opinions on the best method to achieve adequate lighting for opaque samples.

Contextual Notes

Participants express uncertainty regarding the effectiveness of their proposed solutions, and there are assumptions about the size and nature of the samples that may affect the outcomes.

Who May Find This Useful

Individuals working with optical microscopes, particularly those examining opaque materials, may find the discussion relevant.

refind
Messages
50
Reaction score
0
I have an optical microscope and some opaque samples that I want to examine the surface of. I drew up a picture of how the microscope is arranged (attached). There is a series of lenses on a rotating drum that are located underneath the stage where glass slides are placed. The lenses usually come very close to the glass, almost touching it.
I'm having a lot of trouble lighting up the surface of the sample I need to look at. There needs to be quite a bit of light in order to get any decent image, and there is no space underneath to shine light from below. I have an optical fiber illuminator (brand Fiberlite) and it's still too big to snake through underneath. The lenses usually block all the light.
I tried to put aluminum foil on the bottom of the glass slide and use it to reflect the light fom above but that failed too, there was simply not enough light. I'm stuck right now and need some help.

Any ideas?
 

Attachments

  • WP_000160.jpg
    WP_000160.jpg
    21.7 KB · Views: 557
Engineering news on Phys.org
Can you get a thinner sample? Or a smaller one in general, to get more light from the sides? The aluminium foil is a nice approach, but it works best if the sample is smaller than the height of the glass (+- some factor of 2).
 
You can use any opaque surface that is small in size and can reflect the light. It may be a metal.
_________________
biobank information
 
If the microscope is binocular, try removing one eyepiece and shining your light source down that.

This worked for me the other day to give me reflected light when I only had transmitted to work with.

Otherwise, I think you might need to find a better microscope.
 

Similar threads

  • · Replies 25 ·
Replies
25
Views
4K
  • · Replies 7 ·
Replies
7
Views
3K
  • · Replies 4 ·
Replies
4
Views
2K
  • · Replies 4 ·
Replies
4
Views
5K
  • · Replies 3 ·
Replies
3
Views
2K
  • · Replies 0 ·
Replies
0
Views
749
Replies
1
Views
3K
Replies
2
Views
3K
Replies
1
Views
3K
  • · Replies 2 ·
Replies
2
Views
3K