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What is the best sterilization method for this growing medium?

  1. Jul 19, 2010 #1
    I have a 1l solution of Murashige and Skoog micro- and macro-nutrients. It also contains the vitamins and some hormones. The complete list is copied below. I emphasized the compounds that I think are the most unstable ones. Are the compounds listed autoclavable? I have anecdotal reports of only IAA not being autoclavable. Can you help me?

    I want to sterilize this solution. Should I go with an autoclave? If so, for how long? Maybe UVC sterilization? Filter sterilization is not an option since I don't have access to a well-equipped lab.

    Micro-nutrients:
    * Ammonium nitrate (NH4NO3) 1,650 mg/l
    * Boric acid (H3BO3) 6.2 mg/l
    * Calcium chloride (CaCl2 · 2H2O) 440 mg/l
    * Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
    * Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l
    * Cupric sulfate (CuSO4 · 5H2O) 0.025 mg/l
    * Potassium phosphate (KH2PO4) 170 mg/l
    * Ferrous sulfate (FeSO4 · 7H2O) 27.8 mg/l
    * Potassium nitrate (KNO3) 1,900 mg/l
    * Manganese sulfate (MnSO4 · 4H2O) 22.3 mg/l
    * Potassium iodide (KI) 0.83 mg/l
    * Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l
    * Zinc sulfate (ZnSO4·7H2O) 8.6 mg/l
    * Na2EDTA · 2H2O 37.2 mg/l

    Macro-nutrients:
    * i-Inositol 100 mg/l
    * Niacin 0.5 mg/l
    * Pyridoxine · HCl 0.5 mg/l
    * Thiamine · HCl 0.1 mg/l
    * IAA 1–30 mg/l
    * Kinetin 0.04–10 mg/l
    * NAA
    * BAP
    * Glycine (recrystallized) 2.0 g/l
    * Sucrose 20 g/l
    * Agar 10 g/l
     
  2. jcsd
  3. Jul 19, 2010 #2

    Andy Resnick

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    Science Advisor
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    I would expect UV sterilization to degrade the nutrients. I would just filter the solution through a 0.22 um pore filter- it's really not that difficult to set up. AFAIK, none of your nutrients will stick to the filter.
     
  4. Jul 19, 2010 #3
    also, the mfgr's of your macro nutrients most certainly can tell you how, or even supply you with previously-sterilized quantities
     
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