What type of adhesive to use with Aspergillus Niger experiments?

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SUMMARY

The discussion focuses on selecting an appropriate adhesive for constructing a lab system to grow Aspergillus Niger on solid agar. Mark seeks non-toxic adhesives that do not contain anti-fungals or harmful chemicals. Protein glues such as hide glue, rabbit skin glue, and fish glue are recommended, but they are not suitable for high temperatures or autoclaving. Alternatives like physical attachments or pressure fit systems are also suggested to avoid the need for adhesives altogether.

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  • Knowledge of agar composition and properties
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  • Research non-toxic adhesives suitable for laboratory use
  • Explore physical attachment methods for agar-based systems
  • Investigate the properties of Czapek Dox agar and its applications
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Laboratory technicians, microbiologists, and researchers involved in mold cultivation and laboratory equipment design will benefit from this discussion.

WarpedWatch
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Greetings,

I'm trying to design a small lab system that grows Aspergillus Niger on a solid agar base and I've been thinking of making it from acrylic and using some sort of adhesive to hold it together, but I know some adhesives contain anti-fungals, etc. or they just happen to contain a lot of nasty chemicals that might make my precious molds oh-so unhappy. Does anyone here have laboratory experience with such adhesives, who can suggest the best thing to use? The entire unit will be about 30cm long by about 4cm wide and maybe 4 cm tall with about 20 to 30 small compartments in it, so the glue doesn't have to be super-cheap but I can't afford $100 a drip, either.

thanks for your help,
Mark
 
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Protein glues are non-toxic - hide glue, rabbit skin glue, fish glue are all examples. The only problem is withstanding heat & autoclaving. They don't. Many of these glues have severe adhesion problems above 45C.

Have you considered a physcal attachment - i.e., embedding something like a plastic twist tie in the agar, to allow tying off? 1.7% - 2.0% agar can take some flexing before it breaks. Czapek Dox agar is typically about 1.2%, so, this might not work. I dunno.
 
Why use glue at all? Can't you just use a pressure fit cylinder with an O-ring. Just press the molded agar out when ready. Use a large diameter sliced open plastic syringe as a model of what I'm thinking about.
 

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