Discussion Overview
The discussion centers around the selection of an appropriate negative stain for imaging a sensitive large molecular complex using electron microscopy (EM). The context includes considerations of ionic strength, specimen integrity, and the limitations of conducting the experiment at room temperature without a cold specimen holder.
Discussion Character
- Debate/contested, Homework-related
Main Points Raised
- One participant describes the need to choose a negative stain that maintains the integrity of a large molecular complex under low ionic strength conditions for electron tomography.
- Another participant questions whether the original post is a homework question, suggesting that it may require more effort from the poster to receive help.
- A subsequent reply reiterates the homework question concern, indicating a lack of willingness to assist without further input from the original poster.
Areas of Agreement / Disagreement
Participants do not reach a consensus on the best negative stain to use, and there is disagreement regarding the appropriateness of the original post as a homework question.
Contextual Notes
The discussion does not provide specific recommendations for negative stains or address the implications of conducting the experiment at room temperature.