Which Negative Stain is Best for Sensitive Large Molecular Complexes in EM?

AI Thread Summary
The discussion centers on preparing a sensitive large molecular complex for electron tomography imaging, specifically using low ionic strength solutions due to the complex's integrity being affected by ionic strength. The user plans to collect a dual tilt series of 120 images but faces the challenge of lacking a cold specimen holder, necessitating room temperature experimentation. The main inquiry is about selecting the appropriate negative stain for specimen preparation, with a focus on the implications of the choice. The conversation also touches on the nature of the question, with some participants questioning whether it is a homework assignment and suggesting that effort in formulating the question could lead to better assistance.
i_a_n
Messages
78
Reaction score
0
I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. And my goal is image this by electron tomography by collecting a dual tilt series of 120 images. Other experimental constraints are that I lack a cold specimen holder and thus must to this experiment at room temperature. From the possible negative stains that are available generally, which should I choose to prepare my specimen and why?
 
Last edited:
Biology news on Phys.org
You forgot to change the last "your" to "my". Is this a homework question?
 
Pythagorean said:
You forgot to change the last "your" to "my". Is this a homework question?
Yes. But can you help me somehow?
 
No, but someone who would be able to help you would probably appreciate some effort in answering the question on your part.
 

Thread 'Australian Funnel-web spiders and delta-hexatoxin (aka Versutoxin)'

https://www.discovermagazine.com/the-deadliest-spider-in-the-world-ends-lives-in-hours-but-its-venom-may-inspire-medical-miracles-48107 https://en.wikipedia.org/wiki/Versutoxin#Mechanism_behind_Neurotoxic_Properties https://www.sciencedirect.com/science/article/abs/pii/S0028390817301557 (subscription or purchase requred) he structure of versutoxin (δ-atracotoxin-Hv1) provides insights into the binding of site 3 neurotoxins to the voltage-gated sodium channel...
View full post »

Thread 'Here comes COVID-19 version BA.2, BA.4, BA.5,...'

Popular article referring to the BA.2 variant: Popular article: (many words, little data) https://www.cnn.com/2022/02/17/health/ba-2-covid-severity/index.html Preprint article referring to the BA.2 variant: Preprint article: (At 52 pages, too many words!) https://www.biorxiv.org/content/10.1101/2022.02.14.480335v1.full.pdf [edited 1hr. after posting: Added preprint Abstract] Cheers, Tom
View full post »

Similar threads

Mathematica This Week's Finds in Mathematical Physics (Week 241)
Replies
8
Views
5K

Hot Threads

Recent Insights

Back
Top