Why is our gel electrophoresis gel sticking to the sides and ripping?

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SUMMARY

The discussion centers on the issue of 1% agarose gels sticking to the sides of the mold and ripping during removal, affecting DNA purification processes. The protocol involves using 0.6g of UltraPure low melting point agarose in 60mL of 0.5x TBE buffer from Fisher, with the addition of 1μL of EtBr. Despite following a previously successful protocol, the user experiences consistent failures in a new lab setting. Recommendations include allowing the gel to cool longer and gently lifting the comb from one side to introduce air, as well as contacting the manufacturer for further assistance.

PREREQUISITES
  • Understanding of gel electrophoresis principles
  • Familiarity with agarose gel preparation techniques
  • Knowledge of TBE buffer composition and usage
  • Experience with handling laboratory equipment and protocols
NEXT STEPS
  • Research best practices for agarose gel cooling times
  • Investigate techniques for preventing gel adhesion to molds
  • Learn about the effects of temperature on gel consistency
  • Contact manufacturers for troubleshooting advice on gel preparation
USEFUL FOR

This discussion is beneficial for biophysics graduate students, laboratory technicians, and researchers involved in DNA purification and gel electrophoresis techniques.

jesswess
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I am a biophysics grad student at Michigan State, and the lab I am working in has just started making 1% agarose gels for DNA purification. The problem is when we try and remove the comb from the gel and the gel from the walls of the form, it sticks to the sides, ripping the gel and rendering it unusable. The protocol that we are following as worked for my PI in other labs, but never for myself (or my PI) in this lab. All our equipment, chemicals, and glassware are new because this is a new lab.

So, have any of you had a similar problem? What do you think could be causing this?

I have made sure that it is not the agarose or TBE buffer that is contaminated.
Here is the protocol:
Add 0.6g of low melting point LMP Agarose (UltraPure brand) to 60mL of 0.5x TBE buffer (Fisher brand). Microwave until just boiling and mix until all the agarose is dissolved. Allow to cool, add 1μL of EtBr and pour into mold. Wait ~1 hour and remove gel from mold.

Like I said, my PI used this protocol all the time at his past lab, and he never had this problem.
Thanks so much for your help.
 
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Have you tried letting it sit for a little longer? The only other thing I can think of is putting it in the fridge for the last 15 min. or so.
 
I made hundreds of 1% gels, also on new equipment, and have never had that happen. Be gentle when removing the comb, it helps to start the lifting from one side so that air can come into it. Otherwise contact the company for advice.