Recent content by Lindsayyyy

Hello everyone, I want to immobilize beta-glucosidase (on Sepabeads with an amine group). The problem I have is I don't have a strong chemical/biological background. The immobilization should happen with glutaraldehyde but I can't find an appropiate paper with a protocl I can use. In every...

thank you very much, I will take a look

thanks for your answer. Basically I can detect a typical FRAP "spectrum" but I don't know if I can evaulate correctly because of the problems mentioned above (no full 'cell' ROI) and also no background is available to measure.

Hello everyone, I have the following question: I'm doing FRAP in water with a quite small molecule in order to get the diffusion coefficient. I'm quite new to FRAP but I have a feeling that this doesn't work very well. I couldn't find any papers or publications in general about someone doing...

Hello everyone, I have a question concerning the following: There is a relationship for diffusion concerning the RMS: <x^2>= 2\cdot D \cdot T for one dimension and there is also Fick's 2nd Law. When does the above formula apply? What I don't understand is that in the RMS formula there is...

Hello everyone, I'm quite new to lab work and I have the following question: I'm doing immobilisation on enzymes and I want to quantify how much enzymes is bound to my material after immobilization. I'm using UV-VIS for this. Before the experiment itself I want to a calibration with known...

Alright, I got it now. Thank your very much for your help

So the dot product of T and S0 in my first attachment is negative and the dot product of T and S' is positive? If that's the case I think my textbook is wrong :o

I know when the angle is between 0 and 90 degrees the dot product is positive. for 90 to 180 degrees it's negative. This may sound stupid, but I have a problem understanding this when I take a look at the sketch because the tip of the S0 vector touches the T vector. How do I cope with this? It's...

I think it's positive. I didn't create the sketch, I took it out of my textbook. I still don't understand why dS2 is negative.

Hi everyone Homework Statement I'm trying to understand the Laue equation but I have problems unterstanding the derivation, especially one part in my textbook about the path difference (see attachment) The Attempt at a Solution My textbook says that the path difference here is...

Ok thanks, I try it tomorrow, I tried it now, but I can't concentrate anymore. Thank you very much.

Well, I'm too stupid to understand your approach Ray Vickson, thanks anyways. I calculated my first integral now and come to the same solution you go Ray \int \limits_{-1}^{1+t} (1-\tau+t)(1+\tau) d\tau = \frac 1 6 (t+2)^3 but I have difficulties in the interval where t is bigger than...

Thanks to both to you, I have to go to the university soon, I'll take a closer look at yours then Ray. @Vela, I should have mentioned, that I found this Applet online, that's why both triangle looks shifted. But it's not clear to me where I have to multiply the functions.

Ok, I know my problem now, I don't know which values of h and x get multiplied. I uploaded a picture. When I convolve do I just multiply the values which are above each other at t (in this picture the yellow line) or do I have to multiply every value (like I marked the two orange ones)...