Recent content by mountain

Thanks moonbear! It means a lot to me with what you said and your understanding. I am so lost in his evilness. The day i found out that he changed my filter to make me go crazy i almost burst into tears and wanted so badly to quit and find another lab. But i kept going on with my work and...

Thanks for the replies. I have experienced that after stripping then one of my filters lost all of the signals even the MW marker. I wonder if stripping can do this as a side effect? Yesterday i stripped again and then reprobed with B actin and this time i have got the signals back. Have...

Thanks for your great help. :approve: Right now you are my major source for help. I am facing my worst nightmare in science ever. My advisor is a very bad person. When i do something wrong then he blames on me. He is there to make things worse than helping me out of my myseries. He even...

Yeah, i have an advisor, but he is the worst advisor i ever seen. He does not know it that is why i have to bring it here and hopefully and luckily some of you have experienced it. What do you mean with rinsing the wells? THe gels i use are precast do they still need rinsing because we have not...

Some of the bands in my filter do not have a straight line compared to other bands even if they all are the same proteins. What can be wrong? One end of the band seemed to move faster than the the other end. :cry: Thanks for any ideas!

Dear everybody, Please help me out. I am doing an experiment where i use B actin as the normalizer. The problem is after i have stripped the filters then some of the wells did not have the B actin signals. Even if the intensity of the target protein in that well had the same strength as...

After I have stripped the filters then one of my filters lost all the signals even the signals of the MW marker. What is wrong? The protocol for stripping is this: Warm the stripping buffer (62,5mM Tris-HCl pH 6.7 and 2% SDS) to 50C and put the filter in it for 30min at Room Temp (RT). Wash...

Is that okey that I incubate the filter with primary antibody two overnights before adding secondary antibody for detection? This is because I do not have the equipment to detect the signals in the weekend so I have to detect it on a Monday. Due to it I incubate the filter with primary antibody...

When I make the standard curve then each standard solution is prepared like this: 800ul water 20ul each standard solution 200ul dye concentrate The samples are prepared like this: 800ul water 4ul each sample solution 200ul dye concentrate I wonder if I for instance get 0.5mg/ml on...

What do you mean with " it is the same wherever it is found"? In regarding to its size or what?

I am facing a problem where i am not sure if a pituitary specific protein like for example folate receptor alpha subunit has the same function, size and structure in any locations of a human body. as i know a gene can have different start and end signal which means a transcript will end in...

Sorry, moonbear! I forgot the main thing. :grumpy: i am looking for the size (Da) of the human pituitary glucocorticoid receptors, but what i get is only the human glucocorticoid receptors. my question: is the size and the structure of human pituitary glucocorticoid receptors the same in any...

i am looking for some human pituitary proteins for example glucocorticoid receptor. the problem is i get only the answer for human glucocorticoid receptor and not glucocorticoid receptor from human pituitary. as i know glucocorticoid receptor is different in different human locations, then how...

I have tried to search for links about testing for homoschedasticity and could not find any useful ones which explain what it means and how i can test for it. :cry: Hope for any inputs. Thanks.

The difference between a primer and a PCR product is that the PCR product is a double stranded DNA while the primer is just a singel strand. So should i calculate Tm for the PCR product like as it is a single strand?