Adding salt and water to fibroblast growth medium

  • Thread starter TESL@
  • Start date
  • #1
122
8

Main Question or Discussion Point

Hello,

Do you know the total dissolved particle molarity of fibroblast growth media, and could I achieve to keep the osmotic pressure constant while adding both salt and water to it? (I am assuming the salt does not pass through the cell membrane)

Thank you.
 

Answers and Replies

  • #2
183
5
This one is 270 +/- 10 mOsm/L. I'm not sure why, but there's a bit of variance in human cell culture medias. I found a couple examples of stem cell cultures that ranged from 292 up to 340. In any case, you should have the literature on your specific media or you can contact the manufacturer. Whatever it happens to be, your saline should match the media should match intracellular osmolarity (although the 340 kind of throws me off considering I was taught 295 as physiological).
 
  • #3
122
8
Thank you aroc91!
 

Related Threads on Adding salt and water to fibroblast growth medium

Replies
4
Views
14K
Replies
6
Views
3K
Replies
11
Views
4K
  • Last Post
Replies
12
Views
22K
  • Last Post
Replies
1
Views
2K
  • Last Post
Replies
2
Views
3K
  • Last Post
Replies
2
Views
2K
  • Last Post
Replies
2
Views
4K
Replies
13
Views
2K
Top