For whatever reason, the bio experiment I need to analyze was done with respect to two controls. The "treatment 1" organisms were observed with respect to the control organisms to yield one set of treatment 1 and control data. Then, the "treatment 2" organisms were observed with respect to the control organisms to yield treatment 2 data and a <i>different</i> set of control data. I tried ANOVA, but I didn't know how to account for the fact that two of the treatments were actually the same thing (control). Can I take the difference between control and treatment for both sets of data and do a two-sample t-test or am I stuck with descriptive statistics?