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Biochemistry: Tryptophan fluorescence

  1. Oct 6, 2014 #1
    1. A 100-residue globular protein contains two Trp residues, and has two segments with the same 9-amino acid sequence, SSSLWLAAA. One segment is residues 1-9, and one segment is residues 44-53. What would the complete fluorescence spectrum of the protein MOST LIKELY contain?

    a) It would contain a single band at 350 nm
    b) Two bands, one at 350 nm, and one at 320 nm
    c) It would contain a single band at 320 nm
    d) it would contain a single band at 335 nm

    2. This is all info from my biochem lecture, so I'm not sure what to include other than when Trp is "buried" in the protein it has a wavelength of 320 nm and when it is on the outside of the protein it has a wavelength of 350 nm.

    3. The attempt at a solution: I was thinking that because Trp is surrounded by hydrophobic amino acids it is likely to be buried in the protein, so my answer is c). I dont know the answer to this question though
  2. jcsd
  3. Oct 11, 2014 #2
    Thanks for the post! Sorry you aren't generating responses at the moment. Do you have any further information, come to any new conclusions or is it possible to reword the post?
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