Biology Lab Microscope Magnification Calculations

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SUMMARY

The discussion focuses on calculating magnification for a compound microscope used in a biology lab, specifically for a blood sample examination. The total magnification (Mtotal) is derived from the product of the objective magnification (Mobjective) and the eyepiece magnification (Meyepiece). Key equations include Mtotal = Mobjective * Meyepiece, Mobjective = -di/do = -di/fobjective, and Meyepiece = N/feyepiece. The focal length of the objective lens and the distance between the blood sample and the objective lens are critical calculations for accurate microscopy.

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  • Understanding of compound microscope principles
  • Familiarity with magnification equations
  • Knowledge of focal lengths in optics
  • Basic algebra for substitution in equations
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Students in biology or optics courses, educators teaching microscopy techniques, and laboratory technicians involved in biological sample analysis.

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Homework Statement


To examine a particular blood sample in biology lab, a student uses a compound microscope set to have a magnification of -4530. The objective and eyepiece are both attached to a tube which is 18 cm in length and the eyepiece has a focal length of 2.62 cm. The near point of the person using the microscope is 24 and you can assume she can view the image produced with a fully relaxed eye.

a) What is the focal length of the objective?

b) What is the distance between the blood sample and the objective lens?


Homework Equations


Mobjective = -di/do = - di/ fobjective

Meyepiece = N/feyepiece

Mtotal = Mobjective*Meyepiece


The Attempt at a Solution



i tried using these equations...but they didnt work out for me.. unless I am using the wrong equation... i got them equation from compound microscope section of a book
 
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The equations you have are right; you just have to do some substitution. Starting with your most general/biggest one, Mtotal = Mobjective*Meyepiece, sub in the equations that you have for the Mobjective and Meyepiece. Then, plug in the values given by the problem. You will also need to remember that L(length) = fobjective + feyepiece where the f=focal length. Hope this helps! :)
 

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