Biotechnology and bioluminescence

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SUMMARY

The discussion centers on the concept of introducing bioluminescent genes into roadside trees to create natural streetlights, potentially reducing electricity consumption. Participants highlight the challenges of engineering a power source for bioluminescence, specifically the need for sugar routing and the complexity of integrating luciferin and luciferase within the tree's biology. While the idea is theoretically possible, practical implementation faces significant hurdles, including the production of necessary enzymes and the impact on the tree's oxygen output.

PREREQUISITES
  • Understanding of genetic engineering principles
  • Knowledge of bioluminescent organisms and their biochemical pathways
  • Familiarity with plant biology and metabolic processes
  • Basic concepts of enzyme kinetics, particularly the Michaelis-Menten model
NEXT STEPS
  • Research the mechanisms of bioluminescence in organisms, focusing on luciferin and luciferase interactions
  • Explore genetic engineering techniques for introducing foreign genes into plant genomes
  • Investigate methods for metabolic engineering in plants to optimize sugar routing
  • Study the environmental impacts of genetically modified organisms (GMOs) in urban settings
USEFUL FOR

Biotechnologists, genetic engineers, environmental scientists, and urban planners interested in sustainable lighting solutions and the application of genetic engineering in ecological contexts.

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the other day, I was learning basic bio tech(of 10th grade) .. and found out about genetic engineering.

So what I thought was .. there are these bioluminescencent(light giving) organsisms in the deep sea and in trees too(fire flies!) which must be having some or the other gene to make them produce light. So why not introduce this gene into roadside trees and make them as streetlights?

Then no electricity waste (and though street lights are run on solar panels silicon or other metalloids are wasted here)... instead there will be a multi-benefit of more trees , oxygen , rain ...etc.

This is just what I believe might be possible... please be free and correct me if I am wrong.
 
Biology news on Phys.org
Anyone please guide me whether this is right or not..
 
Man that's an awesome idea.
The only problem I can think of is that most bioluminescent molecules require some form of chemical energy to light up, so you would have to engineer that "power source" into the tree. It could be something simple like routing sugar to the area of luminescence, but then it's also kind of tricky to figure out where to make the luminescent protein in the tree. Hmm, I'm thinking if you could introduce a bioluminescent gene to pine cones that might be feasible?
Theoretically Possible? YEAH
Actually doing it? might be tough.

Haha I just realized I said it would be "simple" to reroute sugar to a specific place. I don't know too much plant science but I'm guessing that aint right.
 
Last edited:
Kglocc said:
Man that's an awesome idea.
The only problem I can think of is that most bioluminescent molecules require some form of chemical energy to light up, so you would have to engineer that "power source" into the tree. It could be something simple like routing sugar to the area of luminescence, but then it's also kind of tricky to figure out where to make the luminescent protein in the tree. Hmm, I'm thinking if you could introduce a bioluminescent gene to pine cones that might be feasible?
Theoretically Possible? YEAH
Actually doing it? might be tough.

Thanks!
You are right though... practically it doesn't exactly work out.
I myself found another disadvantage to this too.

Originally form http://www.photobiology.info/LeeBasicBiolum.html

How Does Bioluminescence Work? All bioluminescence reactions involve an oxygen oxidation of an organic molecule (called the luciferin). The reaction is catalyzed by an enzyme called a luciferase and in many cases, the bioluminescence intensity is assumed to reflect the velocity of the enzyme-substrate reaction, and this intensity is used to analyze the kinetics on the Michaelis-Menten model (Figure 5A). It was first a puzzle that the bioluminescence of aequorin and subsequently of several other like organisms, was found not to involve oxygen kinetically, and these proteins were labeled "photoproteins" (Figure 5B). It was eventually established that the oxygen had already bound to the luciferin, and the photoprotein therefore could be more accurately thought of as a luciferase binding a stabilized reaction intermediate, a peroxy-luciferin.

Many bioluminescent reactions in vitro require cofactors in addition to oxygen, e.g., ATP and Mg2+ for the firefly, Ca2+ for photoproteins (1, 2, 4). In the animal itself (in vivo), there are additional proteins involved for production and regulation, some called "accessory proteins", examples being the fatty acid reductase group of enzymes that produce the bacterial luciferin, a long-chain aldehyde, and there are luciferin-binding proteins in the dinoflagellate and Sea Pansy bioluminescence systems. Also, there are "antenna proteins" that act to modulate the color of bioluminescence, the famous Green-fluorescent protein (GFP) in the jellyfish, and the Lumazine Protein family in bacteria (4). These are named "antenna proteins" by analogy to proteins of similar function in photosynthesis, except that they act in a reverse sense.

Fig5.png


So here we need this enzyme luciferase to catalyze the oxidation of luciferin(that will be hard to produce at large scale). That itself will take away the oxygen produced by the tree and gives away CO2... and this is to be done somewhere inside the tree... and that's going to be tough.

Well .. looks like I will have to refine this idea of mine.
 

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