biotechnology

  1. Ygggdrasil

    New CRISPR-based tool for find-and-replace editing of DNA

    Over the past half decade, researchers have envisioned the CRISPR-Cas9 genome editing system as a way to correct pathogenic mutations in patients, but these reserachers face a number of hurdles in developing the technology for clinical applications. While CRISPR is very efficient at cutting DNA...
  2. SanjuktaGhosh

    PCR in Cancer detection

    How is PCR able to detect chromosomal translocations causing cancer? I understand it is possible through FISH but how PCR?
  3. SanjuktaGhosh

    RAPD - close proximity of primers

    I was reading RAPD kit manual by himedia and couldn't understand why the two primers should be at close proximity and in opposite orientation to actually amplify DNA. 'In RAPD analysis, for PCR to occur: a) the primers must anneal in a particular orientation (such that they point towards each...
  4. Ygggdrasil

    The end of mosquitoes?

    Mosquitoes are responsible for spreading a number of human diseases (including malaria, Dengue fever, and Zika virus), and account for nearly half a million deaths per year worldwide. For many years, scientists have been investigating technologies that could help control or even eliminate...
  5. SanjuktaGhosh

    Biotechnology- pUC vectors

    'The MCSs of many vectors such as the pUC series are flanked by sequences complementary to a universal series of primers, the M13 forward and reverse primers. These priming sites are oriented such that extension of the primers annealed to these sites allows sequencing of both ends of an insert...
  6. SanjuktaGhosh

    What is alpha-complementation?

    I am completely stumped by this question. I can't understand what is meant by complementation. Here's an excerpt from Primrose: 'The activity of the enzyme b-galactosidase is easily monitored by including in the growth medium the chromogenic substrate 5-bromo- 4-chloro-3-indolyl-b-D-galactoside...
  7. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in microscopy 1

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in microscopy 1

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  8. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in microscopy 2

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in microscopy 2

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  9. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Dark-field and phase contrast microscopy

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Dark-field and phase contrast microscopy

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  10. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Differential interference contrast and polarization

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Differential interference contrast and polarization

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  11. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Fluorescence and confocal microscopy

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Fluorescence and confocal microscopy

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  12. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Transmission electron microscopy

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Transmission electron microscopy

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  13. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Transmission electron microscopy cont. and scanning electron microscopy

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Transmission electron microscopy cont. and scanning electron microscopy

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  14. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts 1

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts 1

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  15. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts 2

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts 2

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  16. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- GM counting and Scintillation counting

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- GM counting and Scintillation counting

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  17. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Scintillation counting continued

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Scintillation counting continued

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  18. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Autoradiography and RIA

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Autoradiography and RIA

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  19. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Safety aspects and applications

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Safety aspects and applications

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  20. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Introduction and Basic concepts in chromatography 1

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Introduction and Basic concepts in chromatography 1

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  21. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in chromatography 2

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in chromatography 2

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  22. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- LPLC and HPLC

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- LPLC and HPLC

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  23. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Ion-exchange chromatography

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Ion-exchange chromatography

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  24. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Gel-filtration chromatography

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Gel-filtration chromatography

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  25. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Affinity chromatography

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Affinity chromatography

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  26. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Gas-liquid chromatography

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Gas-liquid chromatography

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  27. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in electrophoresis

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Basic concepts in electrophoresis

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  28. Horizontal and vertical gel electrophoresis - YouTube

    Horizontal and vertical gel electrophoresis - YouTube

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  29. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Native gel electrophoresis and SDS-PAGE

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):- Native gel electrophoresis and SDS-PAGE

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
  30. Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods

    Analytical Technologies in Biotechnology by Dr. A. K. Sharma (NPTEL):Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods

    Copyright reserved to Dr. Ashwani K. Sharma and NPTEL, Govt. of India. Duplication punishable offence. Lectures: http://www.nptel.ac.in/courses/102107028/ Syllabus: http://www.nptel.ac.in/syllabus/syllabus.php?subjectId=102107028
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