Calculating Delta Values: Paired t Test

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Using paired t test, how should I pair these results?
To calculate the delta values, should I use: downstream - upstream for both sep and apr ?
or upstream (sep) - upstream (apr) and downstream (sep) - downstream (apr) ?

Thanks
 

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lamlam1 said:
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Using paired t test, how should I pair these results?
To calculate the delta values, should I use: downstream - upstream for both sep and apr ?
or upstream (sep) - upstream (apr) and downstream (sep) - downstream (apr) ?

Thanks

Only the delta for the same day makes sense as the concern is an upper limit of 4 mg/L increase. Not sure why comparing April and Sep as two populations is relevant, seems to me a one tailed t-test on the delta with a null hypothesis that the mean of the delta >4 would be the relevant test
 
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