Perhaps you could give a little more detail.
What is your application?
Are you working with real or complex eigenvalues?
By total, do you mean total number of eigenvalues...as in you are looking for a count?
If that is the case, I would do something like:
E = eig(A);
F = find(abs(E) < c);
size(F)
Where A is your matrix, and c is your "certain value".