Can Mass Spectrometry be a difficult technique?

[maceng7]

I'm just wondering if there are any difficulties that must be overcome to use mass spectrometry. For example if you were to use it with the three isotopes of Carbon ( C12, C13, and C14) where C12 has an abundance of 99%, C13 1% and C14 0.0000000001%. Based on the abundances how would mass spectrometry be difficult? I'm just curious and I couldn't find anything on the internet. Thanks

 

[DrClaude]

What kinds of problems are you thinking about? Misattributing a $^{13}C$ to $^{12}CH$? How do you think that such low abundances would affect the spectrum?