Can retroviruses be used for gene therapy?

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Discussion Overview

The discussion revolves around the potential use of retroviruses for gene therapy, exploring the mechanisms by which retroviruses can be engineered to deliver genetic material into cells. Participants also touch on the broader implications of gene therapy in various applications, including genetic modification in animals.

Discussion Character

  • Technical explanation
  • Exploratory

Main Points Raised

  • Some participants describe retroviruses as capable of being retrained to attach to cells and replace DNA through a process called reverse transcription.
  • There is mention of the use of viral vectors in retroviral gene therapy, specifically referencing the Pol and Gag genes for capsid and delivery systems.
  • Participants note that retroviral, adenoviral, and lentiviral gene delivery systems have been utilized in gene therapy.
  • One participant points out that these gene therapy vectors have been used in cats to introduce genes that result in observable traits, indicating practical applications of the technology.

Areas of Agreement / Disagreement

The discussion does not appear to reach a consensus, as participants primarily share information and observations without resolving any disagreements or uncertainties regarding the efficacy or safety of retroviral gene therapy.

Contextual Notes

Participants do not provide detailed limitations or assumptions regarding the technical aspects of retroviral gene therapy, nor do they address potential risks or ethical considerations associated with its application.

Jarfi
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Hey, I am a second year university student in BioMedical-Engineering and I've been looking to try something new.

DIY is something I have been into for a while, I own and operate a 3d printer and I'm building other projects. So complexity is not really an issue as long as there is information. Is doing something like a DIY medical testing viable or is it not as user friendly? It seems there is no DIY Medical community around, so I am aware that it must be much harder for the "hobbyist" to break his way into it.

What I'm looking for now is testosterone level testing, and other hormones/chemicals that can be analyzed from a blood sample.

If anyone has links, or explanations on weather it's feasible or not then that would be beneficial.

Thanks in advance,

-Jarfi.
 
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Bystander said:

Holy smokes! :woot:

Virus-based
Retroviruses can be retrained to attach to cells and replace DNA. They go through a process called reverse transcription to deliver genetic packaging in a vector.[34] Usually, these devices are Pol – Gag genes of the virus for the Capsid and Delivery system. This process is called retroviral Gene Therapy, having the ability to re-engineer cellular DNA by usage of viral vectors.[35] This approach has appeared in the form of Retroviral, Adenoviral, and Lentiviral gene delivery systems.[36] These Gene Therapy vectors have been used in cats to send genes into the genetic modified animal "GMO" causing it display the trait. [37]
 
Virus-based
Retroviruses can be retrained to attach to cells and replace DNA. They go through a process called reverse transcription to deliver genetic packaging in a vector.[34] Usually, these devices are Pol – Gag genes of the virus for the Capsid and Delivery system. This process is called retroviral Gene Therapy, having the ability to re-engineer cellular DNA by usage of viral vectors.[35] This approach has appeared in the form of Retroviral, Adenoviral, and Lentiviral gene delivery systems.[36] These Gene Therapy vectors have been used in cats to send genes into the genetic modified animal "GMO" causing it display the trait. [37]
berkeman said:
Holy smokes! :woot:
Well worth watching... more than once, even.

https://en.wikipedia.org/wiki/The_Bourne_Legacy_(film)
 
Last edited:
berkeman said:
Holy smokes! :woot:
Virus-based
Retroviruses can be retrained to attach to cells and replace DNA. They go through a process called reverse transcription to deliver genetic packaging in a vector.[34] Usually, these devices are Pol – Gag genes of the virus for the Capsid and Delivery system. This process is called retroviral Gene Therapy, having the ability to re-engineer cellular DNA by usage of viral vectors.[35] This approach has appeared in the form of Retroviral, Adenoviral, and Lentiviral gene delivery systems.[36] These Gene Therapy vectors have been used in cats to send genes into the genetic modified animal "GMO" causing it display the trait. [37]

Obligatory XKCD reference:
t_cells.png

http://xkcd.com/938/
 
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